SOLVED: You decided to prepare a phosphate buffer from solid sodium dihydrogen phosphate (NaH2PO4) and disodium hydrogen phosphate (Na2HPO4) and you need 1L of the buffer at pH 7.00 with a total phosphate concentration (NaH2PO4 Na2HPO4) of 0.100 M. Hint: Phosphoric acid (H3PO4), a triprotic acid, has 3 pKa values: 2.14, 6.86, and 12.4.
SOLVED: You decided to prepare a phosphate buffer from solid sodium dihydrogen phosphate (NaH2PO4) and disodium hydrogen phosphate (Na2HPO4) and you need 1L
VIDEO ANSWER: We are being asked if we need to make 500 liters of 0.2 molar buffer at pH 7 or if we can use a stock solution which consists of one molar of eac…
numerade.com2022年1月23日
Phosphate-buffered Saline Preparation
SOLVED: Create a recipe for 50.0 mL of a phosphate buffered salinesolution containing: 25mM Phosphate, pH 7.4, 140 mM NaCl. You will be provided stock solutions of 0.500 MNa2HPO4, 0.500 MNaH2PO4, solid NaCl and water. Ignore thecontribution of Na from the phosphate buffers inyour salt calculation. The pKa forHPO42-/H2PO4- =6.86, and the molecular weight of NaCl = 58.45g/mol. Using stoichiometry (T chart) find the grams of NaClrequired.
SOLVED: Create a recipe for 50.0 mL of a phosphate buffered salinesolution containing: 25mM Phosphate, pH 7.4, 140 mM NaCl. You will be provided stock solutions of 0.500 MNa2HPO4, 0.500 MNaH2PO4, solid NaCl and water. Ignore thecontribution of Na from the phosphate buffers inyour salt calculation. The pKa forHPO42-/H2PO4- =6.86, and the molecular weight of NaCl = 58.45g/mol. Using stoichiometry (T chart) find the grams of NaClrequired.
numerade.com
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Buffer P1 (Resuspension Buffer) - Miniprep Buffer Mixing — NeoSynBio
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SOLVED: make a pH = 7.4 phosphate buffer solution using potassiumhydroxide (KOH) and phosphoric acid. Starting with 1.0 L of 150 mMphosphoric acid: 1. how much solid KOH should you add to make thedesired pH = 7.4 phosphate buffer solution? 2.Compute theconcentrations of all buffer species.***Can I get CLEARLY written steps? I have made it through theHenderson-Hasselbalch equation:pH=pKa log(salt/acid)=1.59.......I am stuck here.Thank you!
SOLVED: make a pH = 7.4 phosphate buffer solution using potassiumhydroxide (KOH) and phosphoric acid. Starting with 1.0 L of 150 mMphosphoric acid: 1. how much solid KOH should you add to make thedesired pH = 7.4 phosphate buffer solution? 2.Compute theconcentrations of all buffer species.***Can I get CLEARLY written steps? I have made it through theHenderson-Hasselbalch equation:pH=pKa log(salt/acid)=1.59.......I am stuck here.Thank you!
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