Cre-Lox recombination is a site-specific recombinase technology, used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus.

Cre-loxP recombination allows scientists to excise, insert, or invert specific DNA segments with unprecedented accuracy. This works through two key components: a Cre recombinase and loxP sequence recognition sites.

Cre- loxP system is a widely used powerful technology for mammalian gene editing. This system has advantages which is very simple manipulation and do not require additional factors for efficient recombination [1].

A LoxP site is a specific 34 base pair DNA sequence that is recognized by the Cre recombinase enzyme. It is used to manipulate DNA sequences by inducing deletion or inversion of the target DNA flanked by two LoxP sites.

The Cre-lox system, derived from P1 bacteriophage, is a potent and specific system for controlling gene expression. The protein Cre recombinase recognizes 34 bp loxP sites, and the orientation and location of the loxP sites determines how the genetic material will be rearranged.

Mar 2, 2017 · The cre-loxP-mediated recombination system (the "cre-loxP system") is an integral experimental tool for mammalian genetics and cell biology. Use of the system has greatly expanded our ability to precisely interrogate gene function in the mouse, providing both spatial and temporal control of gene exp …

Mar 14, 2025 · Cre-loxP recombination operates through a highly specific enzymatic process that enables targeted genetic modifications. The system relies on Cre recombinase, a member of the integrase family, which recognizes and binds to loxP sites—34-base pair DNA sequences with two 13-base pair palindromic repeats flanking an 8-base pair asymmetric spacer.

Here, we review the latest innovative strategies of the commonly used Cre-loxP recombination system and its combinatorial strategies with other site-specific recombinase systems.

Feb 7, 2024 · Ultimately, we establish a set of 16 orthogonal LoxPsym variants and demonstrate their use for multiplexed genome engineering in both prokaryotes (E. coli) and eukaryotes (S. cerevisiae and Z....

Cre recognizes and recombines pairs of loxP sequences characterized by an inverted repeat and asymmetric spacer. Cre cleaves and religates its DNA targets such that error-prone repair pathways are not required to generate intact DNA products.

LoxP is the wild-type sequence containing two 13-bp-inverted CRE recognition sequences separated by an 8-bp directional spacer. Lox511 is a mutant site differing by a single base in the spacer region. It is recognized and recombined by CRE, …

Oct 20, 2020 · Cyclization recombinase (Cre) is one of the tyrosine site-specific recombinases, which is known to catalyse the site specific recombination event between two DNA recognition sites (LoxP sites). Cre recombinase consists of 343 …

Cre, a 38 kDa protein, recombines DNA between specific 34-bp sequences, called loxP. LoxP consists of a central 8-bp asymmetric sequence flanked by two identical 13-bp inverted repeats. The central asymmetric sequence determines the orientation of loxP sites (Figure 30.2A).

May 19, 2006 · Two of the most exciting and versatile genetic tools designed in the last 30 years are the Cre-lox and FLP-FRT technologies. Both allow the location and timing of gene expression to be closely regulated. This article briefly outlines …

In addition to the generation of subtle mutations, this system allows for a number of other genotypic options in ES cells or mice by strategically incorporating Cre recombinase recognition (loxP) sites into the genome and the subsequent expression of recombinase in vitro or in vivo.

Cre recombinase (Cre) is a 343-amino-acid protein comprised of 4 subunits that recognizes pairs of specific 34 bp DNA sequences called loxP sites. It initially creates a DNA loop and then either excises or inverts the looped segment depending on the orientation of the loxP sites [1 – 3].

Depending on the relative orientation and location of loxP sites, recombination can facilitate different outcomes including the excision, inversion, or translocation of DNA sequences.

LoxP sites are specific sequences of 34 base pairs (bp) consisting of an 8-bp core sequence, where recombination takes place, and two flanking 13-bp inverted repeats [31]. You might find these chapters and articles relevant to this topic. Scott A. Juntti, ... Nirao M. Shah.

Using analytical ultracentrifugation and electrophoresis approaches, we have studied the energetics of Cre-mediated synapsis of loxP sites. We found that synapsis occurs with a high affinity (Kd = 10 n m) and is pH-dependent but does not require divalent cations.

These CRISPRs cut specifically within lox P, an approach that is a departure from previous gene editing in vivo activity detection techniques that targeted adjacent stop sequences.