Cell line U-2 OS is chromosomally highly altered, with chromosome counts in the hypertriploid range. We did not find the hypodiploid cell population described by J. Ponten, et al., Instead, most of the population has slightly higher counts than first described.

U-2 OS is (Fig 1) one of the earliest described cancer derived cell lines. It was obtained from a moderately differentiated bone sarcoma of the tibia of a 15-year-old girl by Ponten and Saksela in 1964. The cell line was originally designated ‘2T’ and is believed to be one of the first human cell lines derived from a mesenchymal tumour 1.

The U2OS cell line, originally known as the 2T line, was cultivated from the bone tissue of a fifteen-year-old human female suffering from osteosarcoma. Established in 1964, the original cells were taken from a moderately differentiated sarcoma of the tibia.

2025年1月4日 · U2OS is a human osteosarcoma cell line widely used in cancer research. It exhibits rapid proliferation and an osteogenic differentiation potential. Despite its aneuploidy, U2OS cells retain wild-type p53. Their application extends to studying osteosarcoma pathogenesis, drug screening, and bone biology.

Human osteosarcoma cell nucleus (U2OS cell line) vitally injected with a Cy3-labelled probe specific for chromosomal telomeric repeat sequences. Note that some spots are relatively large suggesting association or clustering of multiple telomeres.

The human osteosarcoma U2OS cell line is one of the first generated cell lines and is used in various areas of biomedical research. Knowledge of its protein expression is limited and no comprehensive study on the proteome of this cell line has been reported to date.

2022年10月12日 · Here we report the generation and characterization of human cell lines, in which PPO was inactivated using the CRISPR/Cas9 system. The PPO knock-out (PPO-KO) cell lines are viable with the normal...

2020年9月1日 · The results showed that the conditioned medium of osteosarcoma cell line U2OS (U2OS-CM) induces the carcinoma-associated fibroblasts (CAFs)-like transformation of BMSCs and promotes the proliferation, migration and invasion of BMSCs.

2022年4月20日 · Osteosarcoma is the most common primary malignant bone tumor, and U-2OS is a common osteosarcoma cell model. The study obtained a human osteosarcoma U-2OS tool cell line which could stably express Cas9 protein, and …

U2OS is an osteosarcoma cell line derived from a 15-year-old female [4]. Osteosarcoma tumors arise from cells of mesenchymal origin that differentiate to osteoblasts. It is the most common form of bone cancer, responsible for 2.4% of all malignancies in pediatric patients, and its triggers are currently not known [5].