您可点击此处下载Toll-like receptor 4 (TLR4)靶点介绍及实验小贴士pdf文件。 图一：TLR4蛋白结构。 图片来源：SWISS-MODEL。 WB实验贴士. 在类Toll受体中，TLR4是主要的脂多糖 (LPS)受体，并且TLR4被LPS激活。 MyD88-和TRIF-依赖的信号通路被连续触发，并偶联LPS激活的TLR4从质膜到胞内体重新分配。 TLR4在细胞内部的易位及其进一步的溶酶体降解促进了炎症反应的终止。 高表达于胎盘、脾脏和外周血白细胞。 TLR4主要在髓系来源的免疫细胞中表达，包 …

Toll-like Receptor 4 (D8L5W) Rabbit mAb recognizes endogenous levels of total Toll-like receptor 4 protein. Monoclonal antibody is produced by immunizing animals with recombinant mouse Toll-like receptor 4 protein.

66350-1-Ig targets TLR4 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples. PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

wb小妙招： 由于tlr4在人中有多个异构体，并且容易被降解，因此wb检测时可能会出现多条条带。 不同的细胞和组织样本中，tlr4的表达水平和剪切或降解产物可能不同。 由于tlr4容易被剪切和降解，因此在样本中可能检测不到全长的tlr4蛋白，如图8所示。

Cited in 27 publications. View Rabbit Polyclonal anti-TLR4 Antibody - BSA Free (NB100-56580). Validated Applications: WB, Func, ICC/IF, IHC. Validated Species: Human, Mouse, Rat + more. Validated by: Biological Strategies. Sample size available.

30400-1-AP targets TLR4 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples. PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. TLR4, also known as CD284, belongs to the Toll-like receptor family.

Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). This TLR4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide from the Central region of human TLR4. Liquid in PBS containing 0.09% sodium azide, pH 7.3. Store at 4°C short term.

2024年6月17日 · TLR4 Antibody (25) is a mouse monoclonal IgG 1 κ TLR4 antibody, cited in 697 publications, provided at 200 µg/ml; raised against amino acids 198-395 of TLR4 of mouse origin; recommended for detection of TLR4 of mouse, rat, human and canine origin by WB, IP, IF and IHC(P) available conjugated to agarose for IP; and to HRP for WB, IHC(P) and ELISA