M1 and M2 macrophages derived from THP-1 cells differentially …
2015年8月8日 · mRNA and protein expression of M1 and M2 markers confirmed the polarization of THP-1-derived macrophages, which provide a new, easy and well-characterized model of polarized human macrophages.
M1 and M2 macrophages derived from THP-1 cells differentially …
2015年8月8日 · Results: mRNA and protein expression of M1 and M2 markers confirmed the polarization of THP-1-derived macrophages, which provide a new, easy and well-characterized model of polarized human macrophages. Etoposide-induced cancer cell apoptosis was markedly reduced in the presence of THP-1 M2 macrophages, while apoptosis was increased in cells co ...
干货|THP-1分化成巨噬细胞诱导攻略 - 知乎 - 知乎专栏
thp-1细胞是一种人类单核细胞白血病细胞系，常用于研究免疫学和炎症反应。 在实验中，THP-1细胞可以通过特定的刺激剂（如PMA，即磷酸酰肌醇）分化为巨噬细胞， 这一过程广泛应用于以下几个场景： 1.免疫研究：通过…
THP-1（人单核细胞白血病）如何诱导分化？ - 知乎专栏
thp-1（人单核细胞白血病）来自一位急性单核细胞白血病患者的血液，在实验研究中，常常诱导其分化为巨噬细胞和泡沫细胞。 本期普诺赛为大家总结了分化类型及其具体实验操作。
THP1细胞诱导分化巨噬细胞操作手册 - 王进的个人网站
2023年3月30日 · thp-1（人单核细胞白血病）来自一位急性单核细胞白血病患者的血液，在实验研究中，常常诱导其分化为巨噬细胞和泡沫细胞。 1. thp-1分化为巨噬细胞. 巨噬细胞是重要的免疫效应细胞，在固有免疫和适应性免疫应答中发挥重要作用。
实验步骤：我的Thp-1是怎么分化成M1和M2细胞的？ - 知乎
THP-1诱导完成后可通过流式和免疫荧光、ELISA或特异性标记物（如IL1、IL10、精氨酸酶）的基因表达分析来评估向M1和M2型巨噬细胞的正确分化，并且从形态上也可以看出来，都是最基础的鉴定方法，这就不一一述来了。 THP-1诱导并不只是一条”路”，两种方法都可以通罗马.
Activating THP1-derived macrophage in vitro - PMC
Canonical IRF/STAT signaling pathways are activated by IFNs and toll-like receptor (TLR) signaling to make macrophage function toward the M1 phenotype via STAT1. M1 macrophages upregulate IRF5 which is critical for inductions of IL-12, …
THP-1 Monocytic Cells Are Polarized to More Antitumorigenic
2024年6月20日 · CD11b high THP-1 cells treated with PMA and PMA-post-PD were categorized into M0 (HLA-DR low and CD206 low), M1 (HLA-DR high and CD206 low), and M2 (HLA-DR low and CD206 high), resulting in an increased population of M1 macrophages.
Macrophage polarization in THP-1 cell line and primary …
2022年11月1日 · We explored various methods of THP-1 differentiation and suggested several common genes encountered to characterize M1 and M2 macrophages differentiated from THP-1. The systematic review highlights the relevance of using THP-1 derived macrophage as a useful alternative to primary macrophage.
THP-1 and human peripheral blood mononuclear cell-derived macrophages ...
2017年8月1日 · THP-1 may be of greatest use in studies involving M1 polarization. Macrophages (Mφ) undergo activation to pro-inflammatory (M1) or anti-inflammatory (M2) phenotypes in response to pathophysiologic stimuli and dysregulation of the …