STED microscopy - Wikipedia
Stimulated emission depletion (STED) microscopy is one of the techniques that make up super-resolution microscopy. It creates super-resolution images by the selective deactivation of …
受激辐射损耗（STED）显微镜及其应用 - 知乎 - 知乎专栏
STED显微技术作为第一个突破光学衍射极限的远场显微成像技术，其基本原理是采用两束激光同时照射样品，其中一束激光用来激发荧光分子，使物镜焦点 艾里斑 范围内的荧光分子处于激 …
Stimulated emission depletion microscopy - Nature Reviews …
2024年8月8日 · In this Primer, we focus on the most recent advancements in stimulated emission depletion (STED) microscopy, encompassing optics, computational microscopy and probes …
活细胞双色动态超高分辨率成像之STED标记方法 - 知乎
STED (Stimulated emission depletion, 受激发射损耗) 超高分辨率成像是纯光学意义上的超高分辨率技术，成像过程只需打开 STED 激光而无需后期计算，就可以快速得到超高分辨率图像。
STED super-resolved microscopy - Nature Methods
2018年1月29日 · Stimulated emission depletion (STED) microscopy provides subdiffraction resolution while preserving useful aspects of fluorescence microscopy, such as optical …
Deep learning enables fast, gentle STED microscopy
2023年6月27日 · STED microscopy is widely used to image subcellular structures with super-resolution. Here, we report that restoring STED images with deep learning can mitigate …
STED microscopy of living cells - Wiley Online Library
2013年3月19日 · Here, we briefly review the current approaches to super-resolution optical microscopy and present the implementation of STED microscopy for novel insights into live …
STED Nanoscopy Reveals the Ubiquity of Subcortical ... - Cell Press
2015年3月3日 · By harnessing live-cell stimulated emission depletion (STED) nanoscopy and the fluorescent probe SiR-Actin, we show that the periodic subcortical actin structure is in fact …
受激发射损耗显微镜检查 (STED) | Thermo Fisher Scientific - CN
Molecular Probes® CellLight® 试剂是一种靶向特定亚细胞结构的即用型荧光蛋白构建体（GFP 或 tagRFP）。 这些试剂为在活细胞内引入靶向细胞内标记提供了一种简单有效的方法。 线粒体 …
Stimulated emission depletion (STED) super resolution imaging …
2021年3月1日 · STED requires high-intensity depletion lasers together with specific phase modulation optical elements to control the depletion beam morphology, while in contrast, …