Asymmetrical flow field flow fractionation (AF4) is nowadays a common and state-of-the art method for fractionation and separation of macromolecules and particles in a suspension. AF4 is an alternative to HPLC and SEC in cases where column chromatography is …

2021年1月4日 · Asymmetric-flow field-flow fractionation (AF4) has been recognized as an invaluable tool for the characterisation of particle size, polydispersity, drug loading and stability of nanopharmaceuticals. However, the application of robust and high quality standard operating procedures (SOPs) is critical for accurate measurements, especially as these ...

2014年5月6日 · Asymmetric flow field-flow fractionation (AF4) is a widely used and versatile technique in the family of field-flow fractionations, indicated by a rapidly increasing number of publications.

2021年6月1日 · AF4 separation is achieved by establishing a parabolic laminar flow profile of the carrier liquid in a thin channel, without the need for a stationary phase. For this reason, MD-AF4 is a very powerful tool for organic nanoparticles such as liposomes, lipid-based NPs, extracellular vesicles and polymeric particles commonly used in nanomedicine.

2021年1月4日 · In recent years, asymmetrical flow field-flow fractionation (AF4) has attracted increasing attention due to the wide dynamic range (approximately from 1 nm up to 1 μm in the normal mode), and the use of ‘open channels’ without stationary phases or packing materials, which creates a wide range of selectivity in the analytical carrier liquid [19, ...

2021年3月20日 · The development and applications of asymmetric flow field-flow fractionation (AF4) are outlined in comparison with the older and better-known size exclusion chromatography. Recent advances in AF4 instrumentation and predictions for …

2019年3月4日 · We describe the protocol development and optimization of asymmetric-flow field-flow fractionation (AF4) technology for separating and characterizing extracellular nanoparticles (ENPs),...

Asymmetric flow field-flow fractionation (AF4) is a fractionation technique that has gained considerable attention for its gentle separation conditions, broad working range, and versatility. AF4 can be hyphenated to a plurality of concentration and size detectors, thus permitting the analysis of the multifunctionality of nanomaterials.

2020年9月11日 · Asymmetrical Flow Field-Flow Fractionation (AF4) is a powerful technique for sizing of particles in suspension in the size range of approximately 1–1000 nm. There are several ways to derive size information from an AF4 experiment.

2023年5月18日 · Asymmetric-flow field-flow fractionation (AF4) is a gentle, flexible, and powerful separation technique that is widely utilized for fractionating nanometer-sized analytes, which extend to many emerging nanocarriers for drug delivery, including lipid-, virus-, and polymer-based nanoparticles.