60303-1-Ig targets SIRT1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

SIRT1通过催化关键蛋白的去乙酰化来调节细胞凋亡和肌肉分化等过程,在氧化应激和DNA损伤修复中发挥重要作用 (见图1)。 Sirt1的WB检测. 在 Western Blot 实验中，样本准备是实验成功的第一步。 1.裂解样本时加入足量的蛋白酶抑制剂防止蛋白降解。 2.该蛋白高度修饰，分子量达到130kd，可在转膜液中加入终浓度 0.1% 的 SDS 以提高转膜效率。 3.使用 Bradford，Lowry 或 BCA 法对总蛋白进行定量。 每个泳道加入 20-50μg 总蛋白，确保sirt1. 含量在 WB 检测限之上 …

SirT1 antibody detects endogenous levels of total SirT1 protein. The antibody does not cross-react with other sirtuin proteins. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of human SirT1. Antibodies are purified by protein A and peptide affinity chromatography.

SIRT1靶点介绍助您了解其蛋白功能，蛋白定位，蛋白特性及异构体和翻译后修饰。 同时列举了实用的WB实验小贴士，助您实验成功。

SIRT1 regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53, specifically at Lysine 382. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human SIRT1.

SIRT1 Antibody. Applications: WB, IHC, ICC/IF, ELISA, IP. Reactivity: Human, Mouse, Rat, Non-human primate, Chicken. Publications: 367. Images: 189. Clonality: Polyclonal, Monoclonal, Recombinant Monoclonal. Conjugates: Unconjugated, Biotin, CoraLite Plus 670, FITC.

Browse primary antibodies for WB,... Antibodies that detect SIRT1 can be used in several scientific applications, including Western Blot, Immunohistochemistry, Immunocytochemistry, ELISA and Immunoprecipitation. These antibodies target SIRT1 in Human, Mouse, Rat, Non-human primate and Chicken samples.

SIRT1抗体（B-10）是一种小鼠单克隆IgG2b kappa轻链抗体，专门针对人类SIRT1蛋白的氨基酸448-747，有助于通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附测定（ELISA）等方法检测人类样本中的SIRT1。 SIRT1，也称为SIR2、SIR2L1和SIR2alpha，是sirtuin家族的重要成员。 SIRT1拥有一个保守的催化核心，由一个大的Rossmann折叠域和一个小的锌结合域组成，通过一个灵活的连接区域相互连接，该区 …