2017年5月29日 · Here we present single-cell genomic sequencing (SiC-seq), which uses droplet microfluidics to isolate, fragment, and barcode the genomes of single cells, followed by Illumina sequencing of...

单细胞基因组测序（SiC-seq）是第一种基于液滴的方法，被用于表征海洋微生物样本，在该方法中，单个细胞封装在熔融的琼脂糖微滴中，聚合后提供半渗透基质固定细菌细胞，然后在微流体设备中处理这些微凝胶，以生成用于测序的单细胞基因组文库。

SiC-seq (Single-Cell sequencing) is a single-cell microfluidic platform for sequencing the genomes of tens of thousands of cells simultaneously at low coverage. In this workflow, cells from a metagenomic sample are encapsulated in micron-scale hydrogels and individually lysed, tagmented, and merged with a microdroplet containing a unique ...

2017年5月29日 · Here we present single-cell genomic sequencing (SiC-seq), which uses droplet microfluidics to isolate, fragment, and barcode the genomes of single cells, followed by Illumina sequencing of pooled DNA.

2023年12月19日 · 郭博士等人开发了一种单细胞多重测序技术（scCOOL-seq），该技术可以同时分析单细胞染色质状态/核生态位定位、拷贝数变异、倍性和DNA甲基化，这可以指示染色质状态和DNA甲基的不同功能和模式（图1）。 Casasend等人发明了一种地形单细胞测序（TSCS），为细胞提供准确的空间位置信息（图1）。 这项技术在空间上准确地测量和描述了单个肿瘤细胞的特定特征，有助于研究肿瘤细胞的侵袭和转移。 Demaree等人描述了一种高通量和低偏差单细胞 …

2018年5月23日 · In this paper, we describe a method for single-cell genome sequencing (SiC-seq) which uses droplet microfluidics to isolate, amplify, and barcode the genomes of single cells.

Here we present single-cell genomic sequencing (SiC-seq), which uses droplet microfluidics to isolate, fragment, and barcode the genomes of single cells, followed by Illumina sequencing of pooled DNA.

Demaree et al. describe a high-throughput and low-deviation single-cell sequencing (SiC-seq) method that uses droplet microfluidics to separate, amplify, and barcode the genome of a single cell (Table 1). This approach enables broader genomic studies for different cell populations.

2018年5月20日 · SiC-Seq:利用微流体液滴条码进行超高通. 量单细胞基因组测序. 摘要： 大细胞群的单细胞基因组测序应用一直受. 阻于在基因组制备过程中隔离单个细胞的. 技术挑战。 这里我们展示的单细胞基因组测序 （SiC-seq），它使用了液滴微流体技术来分

Here we present single-cell genomic sequencing (SiC-seq), which uses droplet microfluidics to isolate, fragment, and barcode the genomes of single cells, followed by Illumina sequencing of pooled DNA. We demonstrate ultra-high-throughput sequencing of >50,000 cells per run in a synthetic community of Gram-negative and Gram-posit