Cells constantly generate reactive oxygen species (ROS) during aerobic metabolism. The ROS generation plays an important protective and functional role in the immune system. The cell is armed with a powerful antioxidant defense system to combat excessive production of ROS. Oxidative stress occurs in …

2019年4月12日 · In situ mitochondrial ROS amplification is a promising strategy to enhance cancer therapy. Here we report cancer cell and mitochondria dual-targeting polyprodrug nanoreactors (DT-PNs)...

2024年10月15日 · 在目前的基本原理中，常用的细胞内ROS水平检测方法是 DCFH-DA 荧光探针法。 DCFH-DA能够自由穿过细胞膜，在未被氧化时没有荧光。 细胞内的酯酶可将DCFH-DA水解成不能穿过细胞膜的DCFH，从而在细胞内形成积聚。 经过ROS氧化作用后，DCFH会转变成有荧光的DCF，其荧光强度与ROS水平成正比。 一、实验流程. 以下是 流式细胞仪 检测贴壁细胞ROS的实验步骤及注意事项。 实验步骤： 1、准备细胞：将目的细胞均匀铺至六孔板中，实 …

We present a detailed methodology to detect ROS generated as a result of specific FcγR stimulation beginning with macrophage generation, priming, staining, FcγR cross-linking, and ending with flow cytometric analysis.

Oxidative stress results from an imbalance in the production of reactive oxygen species (ROS) and the ability of the cell to scavenge them. ROS react with nucleic acids, proteins and lipids causing cell and tissue damage and can be measured using selective or general indicators.

2018年2月2日 · DCFDA or H2DCFDA (2, 7-dichlorofluorescin diacetate) dye diffuse into the cell and deacetylated by the intracellular esterases as non-fluorescent dye and its oxidized by ROS into green fluorescent compound. The less ROS less fluorescence and high ROS high fluorescence so it can be detected or pictured at excitation at 495nm, emission at 529nm.

2021年6月18日 · Hypoxia is known to stimulate mitochondrial reactive oxygen species (mROS) in cells. Here, we present a detailed protocol to detect mROS using MitoSOX staining in live cells under normoxia and hypoxia. Flow cytometry allows sensitive and reliable quantification of mROS by FlowJo software.

2009年11月12日 · Reactive oxygen species (ROS) are continuously produced in the cell as a consequence of aerobic metabolism, and are controlled by several antioxidant...

ROS level measured by flow cytometry using H 2 DCFDA dye in embryonic stem cells (ESCs) and their differentiated progenies (difESCs): effect of the cell size. (A) Flow cytometry histograms of...

In most cells, mitochondria are the greatest contributors of intracellular reactive oxygen species (ROS). Excess ROS leads to mitochondrial DNA (mtDNA) damage and protein depletion. We have developed a fluorescence-activated cell sorting (FACS)-based protocol to simultaneously analyze RedOx status and mtDNA integrity.