Ribosome profiling, or Ribo-Seq (also named ribosome footprinting), is an adaptation of a technique developed by Joan Steitz and Marilyn Kozak almost 50 years ago that Nicholas Ingolia and Jonathan Weissman adapted to work with next generation sequencing that uses specialized messenger RNA (mRNA) sequencing to determine which mRNAs are being act...

RiboSeq.Org hosts a collection of tools for the processing, analysis and visualization of ribosome profiling data. Explore over 13,000 Ribo-Seq samples in your browser or download processed files for further analysis offline.

2024年11月14日 · RiboSeq.Org enhances the accessibility and utility of public Ribo-Seq data, enabling researchers to gain new insights into translational regulation and protein synthesis across diverse organisms and conditions.

RiboSeq.Org, with its suite of tools including GWIPS-viz, RiboGalaxy, Trips-Viz and now the RDP, together forms a comprehensive ecosystem for Ribo-Seq data analysis and visualisation. This integrated approach offers several key advantages:

In this review we present an overview of such methodologies, outlining their assumptions, data requirements, and availability. At the interface between RNA and proteins, Ribo-seq can complement data from multiple omics approaches, zooming in on the central role of translation in the molecular cell.

2021年9月8日 · Single-cell ribosome sequencing (scRibo-seq) combines nuclease footprinting with small-RNA library construction and a size enrichment to measure translation dynamics in single cells (Fig. 1a).

Ribose-seq allows scientists to determine the precise location and type of ribonucleotides that have been incorporated into eukaryotic or prokaryotic DNA. [1] The technique exploits the presence of the extra hydroxyl groups (OH) found in the 2’ end of ribonucleotides, which can distort and destabilize DNA.

2025年1月6日 · By providing these integrated, user-friendly resources, RiboSeq.Org aims to lower the barrier to reproducible research in the field of translatomics and promote more efficient utilisation of the wealth of available Ribo-Seq data.

2025年3月30日 · Bacterial genomes still contain many hidden and uncharacterized genes. Using complementary Ribo-seq approaches, Froschauer, Svensson et al. here annotate start and stop codons of Campylobacter ...

2019年6月6日 · We present riboStreamR, a comprehensive Ribo-seq quality control (QC) platform in the form of an R Shiny web application. RiboStreamR provides visualization and analysis tools for various Ribo-seq QC metrics, including read length distribution, read periodicity, and translational efficiency.