Ex vivo lung perfusion (EVLP) is a promising procedure for evaluation, reconditioning, and treatment of marginal lungs before transplantation. Small animal models can contribute to improve clinical development of this technique and represent …

2025年4月2日 · Ex vivo lung perfusion (EVLP) enables the maintenance of donor lung function and metabolic activity outside the body, facilitating assessment and potential repair prior to transplantation. 1-3 Multiple centers have established rat EVLP models, 4-6 providing a foundation for animal studies aimed at advancing ex vivo lung repair techniques.

2015年2月25日 · Here, we describe the development of a rat EVLP lung program and refinements to this method that allow for a reproducible model for future expansion. We also describe the application of this EVLP system to model VILI in rat lungs.

2021年12月2日 · In this study, we aimed to develop a stable and reproducible rat EVLP model for prolonged perfusion of pre-injured donor lungs from brain-dead donors. This report details our successfully established protocol for BD and EVLP in rats, and describes tips and pitfalls to facilitate reproduction by other researchers.

2016年12月9日 · Ex vivo lung perfusion (EVLP) is a promising procedure for evaluation, reconditioning, and treatment of marginal lungs before transplantation. Small animal models can contribute to improve clinical development of this technique and represent a substantial platform for bio-molecular investigations.

2024年11月30日 · Drawing upon our practical experience over the past 3 years, our Jiangxi team has systematically refined and summarized the specific details of a rat EVLP experiment in this paper. It encompasses a comprehensive overview of the experimental setup, procedural steps, and potential pitfalls that should be kept in mind.

2024年10月18日 · Ex vivo lung perfusion (EVLP) emerges as a promising technology to evaluate and potentially improve the function of donated lungs. This paper describes the development of a cost-effective and reproducible small animal experimental …

2014年1月14日 · The main objective of this study was to develop a reproducible rat EVLP (R-EVLP) model that enables prolonged evaluation of the explanted lung during EVLP and successful transplantation after EVLP. The donor heart–lung blocks were procured with cold low-potassium dextran solution and immersed in the solution for 1 h at 4°C.

2020年6月25日 · Here, we describe a new rat EVLP model that addresses this need, while enabling the study of lung injury due to cold ischemic time (CIT). The technique involves perfusing and ventilating male Lewis rat donor lungs for 4 h before transplanting the left lung into a recipient rat and then evaluating lung function 2 h after reperfusion.

2019年7月1日 · we describe a new rat EVLP model that addresses this need, while enabling the study of lung injury due to cold ischemic time (CIT). The technique involves perfusing and ventilating male Lewis rat donor lungs for 4 h before transplanting the left lung into a recipient rat and then evaluating lung function 2 h after reperfusion. To test injury