2020年9月15日 · We optimized the Q binary expression system for zebrafish and other vertebrates. QFGal4 is a non-toxic transactivator that can be ubiquitously expressed in zebrafish. We developed QF effector sequences with reduced CpG content to reduce risk silencing. The Q system can be used for gene-tagging and lineage tracing applications.

2020年10月29日 · Here, we present an effective novel strategy for inhibiting translation based on the antisense-controlled formation of an RNA quadruplex-duplex hybrid (QDH) between a G-rich RNA antisense oligoribonucleotide (Q-ASO) and specific mRNA, comprising two distant G-tracts.

2023年9月25日 · Modified mRNA (mRNA) represents a promising new class of therapeutic drug product. Development of robust stability indicating methods, for control of product quality, are therefore crit. to support successful pharmaceutical development. This paper presents the first ion pair reversed phase liq. chromatog.

2018年5月21日 · We optimized a single molecule RNA fluorescence in situ hybridization (RNA-FISH) protocol, which amplifies fluorescence signal to detect candidate transcripts, for use with individual oocytes and...

2021年12月16日 · Here we demonstrate a novel QF-based binary transgene switch (IQ-Switch) that is relatively free of driver toxicity and transgene silencing, and exhibits potent and highly tunable transgene...

QF is a transcriptional activator that binds to the QUAS upstream regulatory sequence to drive reporter gene expression. Unlike Gal4, the QF binding site does not contain essential CpG dinucleotide sequences that are subject to DNA methylation. The QS protein is a repressor of QF mediated transcriptional activation akin to Gal80.

Recently, we have demonstrated the sequence-specific targeting of a 56 nt long EGFR mRNA fragment comprising two distant GGGG tracts by RNA oligonucleotides composed of a chemically modified G ...

2024年5月10日 · QF inhibited mtDNA-cGAS-cGAMP-STING pathway and subsequent inflammatory signaling via regulating the nuclear entry of P65, resulting in the alleviation in fibrosis. Possibly, QF could be served as an effective therapeutic drug in treating bleomycin-induced mouse model that provide a new insight into the prevention of …

2020年10月29日 · Here, we present an effective novel strategy for inhibiting translation based on the antisense-controlled formation of an RNA quadruplex-duplex hybrid (QDH) between a G-rich RNA antisense...

Melting curves for QF-ASO/EGFR and QL-ASO/EGFR were monitored at 260 nm at 295 nm, in a buffer containing 50 mM KCl, 150 mM LiCl and 10 mM Tris-HCl at pH 6.8. from publication: Formation of an RNA ...