2018年2月1日 · The PDZ-SH3-GK (PSG) tandem architecture is evolutionarily conserved from choanoflagelleta, the unicellular ancestor of metazoans, to human (Figure 1 a) [39]. Accumulating evidences suggest that MAGUK PSG tandems form structural and functional supramodules with distinct target binding properties than individual or simple sum of each isolated ...

2020年2月14日 · How such adjacent domains form supertertiary structures and modulate protein–protein interactions has only recently been addressed and is incompletely understood. The postsynaptic density protein PSD-95 contains a three-domain supramodule, denoted PSG, which consists of PDZ, Src homology 3 (SH3), and guanylate kinase-like domains.

2016年3月18日 · This review comprehensively summarizes the mechanistic bases of complex formation between the extended PDZ domain and the extended PBM, as well as the regulated PDZ–target interactions.

2014年11月10日 · We show that the PDZ–SH3–GK (PSG) tandem of PALS1 binds to Crb-CT with ∼100-fold higher affinity than the PDZ domain alone. The structure of the PALS1-PSG–Crb-CT complex provides an atomic picture explaining the exquisitely specific PALS1–Crb interaction afforded by the PSG supramodule.

ZO-1 PDZ3-SH3-GUK Domains —The polypeptide containing the PDZ3-SH3-GUK (residues 417– 803) tandem domains of ZO-1 crystallized in the P3 2 space group and the diffraction pattern exhibited perfect...

2019年3月13日 · PSD-95 MAGUK family scaffold proteins are multi-domain organisers of synaptic transmission that contain three PDZ domains followed by an SH3-GK domain tandem. This domain architecture allows coordinated assembly of protein complexes composed of neurotransmitter receptors, synaptic adhesion molecules and downstream signalling effectors.

2016年8月25日 · In this study, we discover that SynGAP, one of the most abundant PSD proteins and a Ras/Rap GTPase activator, forms a homo-trimer and binds to multiple copies of PSD-95. Binding of SynGAP to PSD-95 induces phase separation of the complex, forming highly concentrated liquid-like droplets reminiscent of the PSD.

突触后密度蛋白PSD-95包含一个称为PSG的三结构域超模块，该模块由PDZ，Src同源性3（SH3）和鸟苷酸激酶样结构域组成。 PDZ结构域与其提议的天然配体的C末端结合，PDZ3结构域（CRIPT）包含CXXC重复序列的相互作用子，仅使用分离的PDZ域的先前实验结果与最简单的蛋白质-蛋白质相互作用场景一致；即两种状态机制。 在这里，我们分析了PSG超模块与CRIPT的结合动力学。 我们显示PSG通过更复杂的机制与CRIPT结合，该机制涉及在第二时标上相互 …

2023年10月23日 · In contrast, the bond between the PDZ3-SH3-GUK (PSG) domain of the cytoskeletal adaptor ZO-1 and the JAM-A intracellular domain becomes dramatically weaker in response to ∼2 pN of load, the amount generated by single molecules of the cytoskeletal motor protein myosin II.

2022年10月10日 · Here, we investigated the interactome of a three-domain supramodule, PDZ3-SH3-GK (PSG) from PSD-95 using bioinformatics to identify potential binding partners, and biophysical methods to characterize the interaction with peptides from these proteins. PSG and the single PDZ3 domain bound similar peptides, but with different specificity.