Suspensions of bacterial cells are exposed to the test substance (liquid or solid) in the presence and in the absence of an exogenous metabolic activation system. At least five different analysable concentrations of the test substance should be used.

471 OECD/OCDE ©OECD 2020 5. The bacterial reverse mutation test is commonly employed as an initial screen for genotoxic activity and, in particular, for point mutation-inducing activity. An extensive data base has demonstrated that many chemicals that are positive in this test also exhibit mutagenic activity in other tests.

The bacterial reverse mutation test (also known as the Ames Assay) uses amino-acid requiring strains of Salmonella typhimurium and Escherichia coli to detect point mutations, which involve substitution, addition or deletion of one or a few DNA base pairs.

Most regulatory bodies require adherence of testing to OECD guideline 471 [17], which implies that confirmation of “negative results” (i.e., apparent absence of mutagenicity of the test material) is expected.

Creative Bioarray provides Bacterial Reverse Mutation Test (Ames test, OECD 471) using 7 Salmonella typhimurium strains and 2 Escherichia coli strains to assess the mutagenic potential of test articles. This assay is available under either Good …

The Ames Mutagenicity Assay (Bacterial Reverse Mutation Assay) is used to detect potential mutagenic activity in chemicals, medical device extracts, and other types of samples. The test is typically performed according to OECD Test Guideline No. 471.

1997年7月21日 · OECD Guidelines for the Testing of Chemicals, Section 4 Test No. 471 ... - OECD - Google Books. The bacterial reverse mutation test uses amino-acid requiring at least five strains of Salmonella...

OECD 471 (adopted 21 st July, 1997); Objective: To establish the potential of the test item to induce gene mutations in bacteria ( Salmonella typhimurium and Escherichia coli) by means of reversions from auxotrophic strains to prototroph. Tester strains: Salmonella typhimurium TA98, TA1537 (for frameshift mutations);

Gene mutations are detected when they cause restoration of the capability of the bacteria to synthesize the essential amino acid that are then able to grow in the absence of the amino acid required by the parent test strain. The assay is performed according to the OECD Guideline 471.

2021年8月10日 · What is the OECD 471 test? The objective of the OECD 471 bacterial reverse mutation test is to detect mutations that reverse mutations present in the test strains and restore the functional ability of the bacteria to synthesise an essential amino acid.