The results showed that there was a single-base insertion in the coding region of Viviparous8 (VP8) in d2003 mutant, which resulted in a premature stop codon. Further genetic allelism tests confirmed that d2003 mutation is a novel allele of VP8.

2013年11月9日 · In this study, we reported the cloning of d2003 gene using positional cloning. The results showed that there was a single-base insertion in the coding region of Viviparous8 (VP8) in d2003 mutant, which resulted in a premature stop codon. Further genetic allelism tests confirmed that d2003 mutation is a novel allele of VP8.

Molecular analysis revealed that Vp8 encodes a putative peptidase closely related to Arabidopsis thaliana ALTERED MERISTEM PROGRAM1. Because the Vp8 regulates meristem development as well as seed maturation processes, including ABA accumulation, we propose that VP8 is required for synthesis of an unidentified signal that integrates meristem and ...

Our results show that maize Vp8 encodes a putative membrane-localized peptidase that is closely related to Arabidopsis AMP1. Loss of Vp8 function causes either lethality or precocious germination of the developing embryo, depending on the genetic background.

Maize viviparous mutants have been demonstrated to have defects in ABA biosynthesis or ABA sensing, and multiple maize vivipary-related genes have been cloned. Both the vp1 and vp4 genes encode a plant-specific transcription factor involved in ABA signaling that can complement the Arabidopsis abi3 mutant allele [ 19 , 20 ].

结果表明，d2003突变体Viviparous8（VP8）编码区存在单碱基插入，导致终止密码子提前终止。 进一步的基因等位基因测试证实，d2003 突变是 VP8 的一个新等位基因。

We show that the viviparous8 (vp8) mutant has a similar pleiotropic phenotype in the W22 inbred background in contrast to the viviparous embryo phenotype exhibited in the standard genetic background, and we confirmed that wpkl is allelic to vp8. Further genetic analysis revealed that the standard vp8 stock contains an unlinked, partially

The maize mutant vp-like8 shows clear viviparous phenotype and stable inheritance, and genetic analysis showed that the mutant phenotype was controlled by a single recessive gene. Using an F 2 segregation population derived from vp-like8 and inbred line Zheng 58, the causal gene was mapped to an interval from 160.4 Mb to 165.6 Mb on chromosome ...

本研究对一个新发现的玉米穗发芽突变 体 (命名为 vp-like8)进行遗传分析和基因定位, 经等 位测验和突变基因的分子鉴定发现, vp-like8 是 Vp1 基因的一个新等位突变体。 该研究为进一步探索玉 米穗发芽的分子遗传机制丰富了实验材料。 粒种植。以此方式连续多代自交, 观察 vp-like8 突变 体的遗传稳定性, 并进行遗传分析。 在每一世代自 交授粉后 30 d, 对 vp-like8 穗发芽的表型进行鉴定, 剥开果穗苞叶, 选取出现穗发芽表型的果穗, 脱粒 并统计正常与穗发芽籽粒的分离比。

We show that the viviparous8 (vp8) mutant has a similar pleiotropic phenotype in the W22 inbred background in contrast to the viviparous embryo phenotype exhibited in the standard genetic background, and we confirmed that wpk1 is allelic to vp8.