The c-Myc (Myc) tag originated from c-myc, a proto-oncogene which encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis, and cellular transformation. The Myc tag is only 10 amino acids long (EQKLISEEDL).

The Myc tag is a small, immunoreactive peptide tag (11 amino acids) that is ideal for co-immunoprecipitation studies and Western blots. It binds to the cMyc Monoclonal Antibody and can be incorporated on the N-terminus, the C-terminus, or internally.

Explore widely-used epitope tags, such as c-myc and HA, including recommended applications and what to watch out for.

2021年7月18日 · The protein tag is a polypeptide or protein expressed as a fusion with the target protein using in vitro DNA recombinant technology, which facilitates the expression, detection, tracer and purification of the target protein.

2023年5月11日 · The Myc tag is a 10 amino acid peptide (EQKLISEEDL) derived from the protooncogene of the same name. The tag has a net negative charge with 4 negatively charged and 1 positively charged amino acids. Myc tags are frequently used for western blot, flow cytometry, and immunofluorescence .

2.1.3 c-Myc tag. The c-myc tag was created from the human c-myc gene product and is composed of 10 amino acids. This epitope tag is recognized by the anti-c-myc monoclonal antibody 9E10 [16]. The c-myc tag can be fused at the C- …

The Myc-tag allows simple and efficient affinity purification of the tagged protein using Myc-tag specific antibody conjugated to agarose-beads. The Myc-tag (EQKLISEEDL-tag) also can be used for detection in western blot by using a Myc-tag-specific antibody.

Myc tag is a small peptide tag that can be fused to proteins for their recognition and detection through Myc antibodies. Myc tag has been successfully applied in many biological assays, including WB, immunoprecipitation (IP), immunofluorescence, pull …

2020年4月24日 · The Myc and c-Myc tag are two terms for the same tag, which is derived from the c-Myc protein and has a peptide sequence of EQKLISEEDL. The tag is used to label target proteins to enable easier identification or purification.

Myc tag A small, immunoreactive tag—ideal for co-IP & western blots; Express pCMV-Myc & pCMV-HA Vector Set »