2025年3月25日 · The interactions between L8 and these beneficial bacteria, and the potential association of their LDH with MRSA T144 CR, warrant more investigation. Mechanistically, we induced the expression of LDH from L8 in E. coli BL21 using homologous recombination techniques to uncover the potential role of LDH-mediated resistance to MRSA T144 ...

2016年11月1日 · The prepared LDH achieved 90% at pH 8.50 which is the pH of wastewater and at the same time exhibited durable antimicrobial activities against MRSA, Gram-negative, Gram-positive and fungi. Results have significant implications in the field of bioremediation of water with little cost, simple operation, high productivity and easiness of the ...

2019年10月17日 · LDH is an important enzyme that catalyses the mutual transformation of pyruvate and lactic acid. It is essential for MRSA to maintain virulence, evade the innate immunity of the host, and form biofilm (Ferreira et al. 2013). LDH assay showed that oridonin could reduce the synthesis of LDH.

2024年2月15日 · In this study, after treatment with 62.5 µg/mL of CA, the MRSA suspension exhibited significantly higher LDH activity compared to the control group, with a noticeable increase in extracellular LDH activity observed between 4 and 12 h.

These LDH-NPs showed significantly improved killing of intracellular MRSA in HEK 293 cells in vitro. Complete eradication of MRSA was found when cells were treated with LDH-NPs, whereas millions of bacteria remained when treated with vancomycin alone.

2024年10月5日 · Infectious disease caused by methicillin-resistant Staphylococcus aureus (MRSA) seriously threatens public health. The design of antimicrobial peptide mimics (AMPMs) based on natural products (NPs) is a new strategy to kill MRSA and slow the development of drug resistance recently.

Objective: We evaluated oridonin effects on MRSA cell membrane and wall, protein metabolism, lactate dehydrogenase (LDH), DNA and microscopic structure. Materials and methods: Broth microdilution and flat colony counting methods were used to measure oridonin minimal inhibitory concentration (MIC) and minimum bactericidal concentration (MBC ...

据报道，用硝酸盐作为层间阴离子可轻松而简单地获得Zn-Fe层状双氢氧化物（LDH）。 共沉淀法产生高结晶度的LDH，其特征在于XRD，SEM，TEM和FT-IR。 结果表明，在pH 11和4 h时，去除了99.8％的Cd + 2。

2025年1月10日 · The LDH assay measures the release of LDH enzyme from cells into the surrounding medium, a marker of cellular membrane integrity and cytotoxicity. An increase in LDH release indicates higher cell damage and death, whereas …

LDH assays were employed to evaluate the cytocompatibility efficiency of PHA/Ch-WS 2 nanocomposites against spontaneously immortalized human keratinocytes (HaCaT) cell lines. Thus, this research presents a simple, facile, cost-effective, and greener route for the fabrication of biopolymer-based nanocomposites, which can be effectively utilized ...