2016年10月14日 · Outer membrane vesicles (OMVs) are nanoscale proteoliposomes that are ubiquitously secreted by Gram-negative bacteria. Interest in these bioparticles has escalated over the years, leading to discoveries regarding their composition, production, and vaccine potential.

In the present study, we have compared the effects of standard OMV isolation methods using detergent or EDTA with those of genetic modifications of LPS to yield a penta-acylated lipid A (lpxL1 and pagL) on the in vitro induction of innate immune responses.

2019年10月7日 · Lipopolysaccharide (LPS) is present in OMVs at a concentration of ∼0.5 mg per milligram of total OMV proteins (14, 26). By binding to TLR4, LPS substantially contributes to the excellent adjuvanticity properties of OMVs. However, LPS abundance is also responsible for OMV reactogenicity, which has to be reduced for human use.

2017年9月1日 · Outer membrane vesicles (OMVs) are naturally non-replicating, highly immunogenic spherical nanoparticles derived from Gram-negative bacteria. OMVs from pathogenic bacteria have been successfully used as vaccines against bacterial meningitis and sepsis among others and the composition of the vesicles can easily be engineered.

2021年9月19日 · Bacterial outer membrane vesicles (OMVs) are nanometer-scale, spherical vehicles released by Gram-negative bacteria into their surroundings throughout growth. These OMVs have been demonstrated to play key roles in pathogenesis by delivering certain biomolecules to host cells, including toxins and other virulence factors.

2014年1月29日 · We evaluated the adjuvant effect of a modified glycoform of lipopolysaccharide (LPS) (LgtB-LpxL1) compared to that of the nonmodified glycoform Lpxl1 serogroup B meningococcal H44/76 native outer membrane vesicles (nOMVs) on immune responses to vaccination with the recombinant meningococcal protein, rPorA, tetanus toxoid, or …

2020年7月9日 · Outer membrane vesicles (OMVs) are valued for their unique, convenient, and amendable functions. They are flexible, controllable nanoparticles, which can be modified in different ways, for use in a wide array of applications like adjuvants, vaccines, drug presentation, and fluorescence tracking.

2016年4月22日 · Gram-negative bacteria are able to produce outer membrane vesicles (OMVs). OMVs are mainly composed of LPS, outer membrane and periplasmic proteins and phospholipids 6. OMVs are formed by blebbing...

2024年12月20日 · Acyltransferases lpxL mutant will cause lower OMVs toxicity. lpxA code UDP-N-acetylglucosamine acyltransferase, but its influence on OMV immune activation is still unclear. and it can also be used as a delivery system for heterologous antigens, which has gained wide attention in the research of OMV vaccine [ 28 – 31 ].

2019年5月29日 · Certain chemical forms of LPS, such as penta-acylated LPS produced by PagL, increase the size and number of OMVs that bacteria release, indicating that LPS modification can stimulate OMV...