Stimulation of mouse macrophages with LPS leads to tumor necrosis factor (TNF-α) secretion and nitric oxide (NO) release at different times through independent signaling pathways. While the precise regulatory mechanisms responsible for these distinct phenotypic responses have not been fully delineated, results of our recent studies strongly ...

2015年1月23日 · Here we examine the inflammatory outcome of TNF- and lipopolysaccharide (LPS)-induced necroptosis and report that this mode of cell death resulted in a dramatic suppression of the production of...

2000年12月22日 · Subcellular fractionation of LPS-stimulated macrophages revealed that LPS signals transduced by Tpl2 specifically promote the transport of TNF-α mRNA from the nucleus to the cytoplasm. Protection from microbial pathogens is mediated by a variety of inducible effector mechanisms that are triggered by these microbes.

为了模拟免疫系统会感染的响应，可以用TNFa、IFNgamma、IL4、IL13、IL17A、IL17F等细胞因子刺激细胞，其中TNFa是单核细胞浸润表皮时产生的，在适应性免疫介入后，Th1响应（细菌病毒感染）中T细胞会产生TNFa、IFNgamma，Th2（湿疹、过敏）响应则对应IL4、IL13，Th17响应（系统性红斑狼疮）对应IL17A、IL17F。 检测的指标方面，可以检测IFNa和IFNbeta这些I型干扰素，也可以检测CXCL10、CXCL12之类的趋化因子，还可以检测IL33、TSLP、IL1beta之类 …

During gram-negative sepsis, human monocytes are triggered to produce large quantities of proinflammatory cytokines such as tumor necrosis factor alpha (TNF-α) in response to endotoxin (lipopolysaccharide [LPS]). Several studies have identified ...

1.提出了一个新的机制性的解释LPS（脂多糖）诱导的 TNF-α （肿瘤坏死因子α）的“疾病模型”，我不太确定在这里使用“疾病模型”是否恰当，或许也可以说为是一个新的机制性的解释LPS（脂多糖）诱导的TNF-α（肿瘤坏死因子α）的 PKPD模型 （药物代谢动力学于药物效应动力学模型）。 TNF-α也有一个分布的过程，并使用2房室模型描述TNF-α的分布。 TNF-α在初始时刻为零，生成速度由LPS影响。 转移室模型的最后一个房室中的量通过一个Emax模型（激活、无基线、S …

The production of TLR-dependent proinflammatory cytokines such as tumor necrosis factor–α (TNF-α) and macrophage inflammatory protein 1α (MIP-1α) is substantially suppressed in the presence of PGE 2 or ISO, whereas the secretion of cytokines associated with anti-inflammatory activity in macrophages, such as interleukin-10 (IL-10) and ...

2005年3月25日 · Previously, we identified a transcription factor, LPS-induced TNF-α factor (LITAF), that regulates TNF-α transcription. We now report the discovery and characterization of a regulatory cofactor that we call signal transducer and activator of transcription (STAT) 6 (B) because of its considerable homology to STAT6 [here referred to as STAT6 (A)].

2015年12月8日 · Lipopolysaccharide (LPS) is currently considered one of the major players in non-alcoholic fatty liver disease (NAFLD) pathogenesis and progression. Here, we aim to investigate the possible role of LPS-induced TNF-α factor (LITAF) in inducing a pro-inflammatory and pro-fibrogenic phenotype of non-al …

2010年1月8日 · In the first part of this study, we identified the TLR4 signalling pathways activated by LPS (NFkappaB and p38 MAP Kinase), which lead to the secretion of TNFalpha by mature human adipocytes. We have also shown that PI3Kinase is implicated in this process.