These results suggest that BV2 microglia activated by LPS triggered TLR4/MyD88/NF-κB signaling pathway that induced the release of IL-1β and could participate in the PC12 cells injury. Microglia play an important role in neuronal protection and damage. However, the molecular and cellular relationship between microglia and neurons is unclear.

2020年1月11日 · LPS significantly decreased Vps34 expression in N9 microglial cells by activating the PI3KI/AKT/MTOR pathway without affecting the levels of lysosome-associated proteins and enzymes. More importantly, overexpression of Vps34 significantly enhanced the autophagic flux and decreased the accumulation of SQSTM1 in LPS-stimulated N9 …

2020年7月16日 · 楼主科研萌新一枚，正在做LPS诱导bv2激活的模型，所用的浓度是LPS跟IFN-gama各100ng/ml和LPS1ug/ml，但NO与流式检测出来与对照组几乎没有差别（平均荧光强度亦无变化），细胞株已换，LPS也重新购入（sigmaL4391）麻烦各位前辈帮忙分析一下原因，谢谢各位 …

2019年7月7日 · 大家好，我刚接触细胞，现在用正在做LPS诱导BV2的炎症模型，我所用的LPS浓度是100ng/ml和1ug/ml，可是这两个检测出来的mtt与对照组几乎没有差别，一氧化氮检测也没有差别，我自己分析了一

2020年1月20日 · To determine whether inflammation triggers lipid droplet formation in microglia, we treated the mouse microglia-derived BV2 cell line with LPS and found a fivefold increase in the number of...

2018年9月19日 · Ibrutinib significantly reduced LPS-induced increases in proinflammatory cytokine levels in BV2 microglial and primary microglial cells but not in primary astrocytes. Ibrutinib regulated TLR4 signaling to alter LPS-induced proinflammatory cytokine levels.

本研究通过观察NaB对脂多糖 (lipopolysaccharide，LPS)诱导BV2细胞表型极化的调节作用，从TLR4/NF-κB信号通路探讨其可能的抗炎机制，探索肠-脑轴理论中肠道菌群代谢物在调控CNS炎症性疾病的价值。 小鼠BV2小胶质细胞 (CL-0439)购自武汉普诺赛生命科技有限公司。 采用DMEM培养基，加入10%胎牛血清和1%双抗；置于37 ℃、5% CO 2...

2021年7月15日 · SDF-1a suppress caspase-1 activity in LPS-induced BV2 cells. The activation of the CXCL12-CXCR4 signaling axis is implicated in the regulation of cell survival, proliferation, and mobilization of bone marrow stem cells into the injured site.

摘要： 目的 探讨京尼平（genipin）对脂多糖（LPS）诱导小胶质细胞（BV-2）炎症及凋亡反应的作用及机制。 方法 采用LPS诱导BV-2小胶质细胞建立中枢神经系统炎症和凋亡反应模型。 实验细胞分为4组：空白对照组、京尼平组、LPS组、LPS+京尼平组。 通过ELISA、RT-PCR、Western Blot、细胞流式检测细胞的炎症及凋亡反应。 结果 与空白对照组比较，LPS可以诱导BV-2细胞上清培养基中IL-6、IL-1β和TNF-α释放和细胞内IL-6、IL-1β和TNF-α转录的增加；同时，LPS还可 …

Our study showed that LPS promoted cell migration via augmentation of Src expression in BV2 cells. Moreover, LPS treatment decreased SET8 expression and upregulated the expression of the transcription factor ETS proto-oncogene 1 (ETS1). Overexpression of both SET8 and small interfering ETS1 reversed LPS-induced Src expression and cell migration.