2019年8月15日 · 这些病例可以通过map（小鼠抗体产生试验）或pcr 检测，或将已知未免疫成年鼠暴露于疑似种群。这些鼠血清转化后可以用ifa、mfiatm 或elisa 进行抗体的检测。 对研究的影响. lcmv 感染对研究最大的干扰是潜在的感染员工的风险。

淋巴细胞脉络丛脑膜炎病毒 (Lymphocytic choriomeningitis virus，以下简称LCMV)是一种球形包膜病毒，直径在60~300nm之间。 螺旋核衣壳包含一个由两个反义RNA片段组成的RNA基因组。 反义RNA与正义mRNA链互补，表明它必须首先被转录成正义mRNA链，然后才能翻译成所需的蛋白质。 LCMV基因组. 病毒有两个反义RNA链： L链和S链。 L链是双义RNA，编码相反方向的多种蛋白质，被一个基因间区域隔开。 它的大小约为7.2kb，编码一个RNA依赖的RNA聚合酶（L; …

We generated a differentiation space map (DSM) of the spectrum of CD8 T cell states in acute and chronic lymphocytic choriomeningitis virus (LCMV) infection across time points using scRNA-seq. Analyses across four time points revealed a linear differentiation trajectory in acute infection, whereas a bifurcation between effector and exhausted ...

2017年9月27日 · We report a fluorescence in situ hybridization (FISH) assay that allows the visualization of lymphocytic choriomeningitis mammarenavirus (LCMV) genomic RNAs in individual cells. We show that viral S segment genomic and antigenomic RNA, along with viral nucleoprotein, colocalize in subcellular structures we presume to be viral replication factories.

MAP (mouse antibody production) testing or PCR testing can be of use in these cases, as can exposure of known naïve adult mice to a suspect colony. These naïve adults will seroconvert, and then IFA, MFIATM or ELISA can be used to detect antibodies.

淋巴细胞性脉络丛脑膜炎（lymphocytic choriomeningitis，LCM）是由淋巴细胞性脉络丛脑膜炎病毒(LCMV)引起的人和多种动物共患的病毒性疾病。 小鼠感染表现为大脑型、内脏型和迟发型3种病型，人感染主要表现流感样症…

Lymphocytic choriomeningitis virus (LCMV) is an enveloped, segmented single-stranded RNA virus of the genus Arenavirus family, Arenaviridae. It can easily be propagated in several commonly used cell lines like BHK-21 cells. However, cells are not lysed and a cytopathic effect (CPE) is not visible.

2022年9月7日 · In this work, we designed a recombinant reporter arenavirus lymphocytic choriomeningitis virus that stably expressed nanoluciferase (LCMV-Nluc). The LCMV-Nluc was proved to share similar biological properties with wild-type LCMV and the Nluc intensity reliably reflected viral replication both in vitro and in vivo .

2021年10月27日 · Lymphocytic choriomeningitis virus (LCMV) is an Old World mammarenavirus found worldwide because of its association with the house mouse. When LCMV spills over to immunocompetent humans, the...

2023年3月30日 · 拉霍亚免疫研究所 (LJI)科学家Erica Ollmann Saphire博士和Shane Crotty博士领导的一项新研究表明，一种抗体 (上图的灰色部分)可以结合并中和LCMV。 多亏了这项新工作，我们现在更接近于设计针对LCMV的疫苗和治疗方法。 他们的论文“抗体介导的淋巴细胞性绒膜脑膜炎病毒中和的结构基础”于2023年3月28日发表在《细胞化学生物学》上。 主要结论: 目前还没有针对淋巴细胞性脉络丛脑膜炎病毒 (LCMV)感染的疫苗或疗法。 这种病原体很容易传播，在世界各 …