An L-ribonucleic acid aptamer (L-RNA aptamer, trade name Spiegelmer) is an RNA-like molecule built from L-ribose units. [1] It is an artificial oligonucleotide named for being a mirror image of …

We show that this approach is compatible with the assembly of several classes of functional l -RNA, which we highlight by generating a 124-nt l -RNA biosensor that functions in serum. As …

2022年4月20日 · RNA G-quadruplex (rG4)-SELEX is a method that generates l -RNA aptamers to target an rG4 structure of interest, which can be applied to inhibit G-quadruplex-mediated …

2022年7月13日 · Our work introduces a novel strategy and reports a new L-aptamer candidate to target APP 3'UTR rG4 structure, which laid the foundation for further applying L-RNA as an …

Spiegelmer (L-RNA aptamer) is a unique class of aptamer that is composed of unnatural L-RNA nucleotides, and so far there are limited L-RNA aptamer candidates and applications being …

L-RNA aptamers were developed that bind to barnase ribonuclease and thereby inhibit the function of the enzyme. These aptamers were obtained by first carrying out in vitro selection of …

2015年11月19日 · The resulting L-aptamers bind L-barnase with an affinity of ∼100 nM and function as competitive inhibitors of enzyme cleavage of D-RNA substrates. L-RNA aptamers …

2024年12月27日 · Ribonuclease L is an endonuclease that is activated as part of the dsRNA-driven innate immune response. Active RNase L cleaves pathogenic RNAs as a way to …

Olea et al. select mirror-image RNA molecules, composed of the non-natural L-isomer, for their ability to bind and inhibit RNase, an enzyme that rapidly degrades natural RNA.

We report 2.8 and 2.1 angstrom crystal structures of human RNase L in complexes with synthetic and natural ligands and a fragment of an RNA substrate. RNase L forms a crossed homodimer …