Nov 1, 2011 · iCIEF is a more suitable technique to assess charge heterogeneities of ADCs. The separation mechanism of this electrophoretic technique is based primarily on pI differences of protein charge variants.

icIEF is a technique to separate proteins based on their isoelectric point. See how Maurice an automated icIEF instrument can simplify protein charge analysis.

Having been amply described already in 1960-1961, how comes that isoelectric focusing (IEF) went largely unnoticed for just about a decade? Well Harry was a free-lance scientist in a …

Experience icIEF separation and UV detection coupled with mass spectrometry identification on the ZenoTOF 7600 system. One workflow, on a single platform, enables rapid monitoring of intact biotherapeutics and identification of charge variants throughout the drug development pipeline.

May 1, 2022 · Capillary isoelectric focusing with whole-column imaging detection (icIEF) technology is now the mainstream technology to perform analytical isoelectric focusing analysis in pharmaceutical industry.

Mar 1, 2025 · We present an integrated protocol for studying the charge heterogeneity of therapeutic proteins combining two workflows: imaged capillary isoelectric focusing (icIEF) with fractionation and online mass spectrometry (MS) detection on a single platform.

Feb 5, 2023 · Since the first commercial imaged capillary isoelectric focusing (icIEF) instrument was developed twenty years ago, the technology has become the gold standard of quality and manufacturing process control in the biopharmaceutical industry.

To support analysis of the charge heterogeneity of USP mAbs, we developed methods for cIEF using the SCIEX platform (PA 800 Plus) and icIEF using Bio-Techne’s Protein Simple Maurice and iCE3 platforms (Table 3).

Feb 5, 2023 · In this work, we developed a robust and flexible icIEF-MS platform which overcomes these challenges to achieve both the rapid icIEF separation and high-resolution MS (HRMS) identification of protein charged variants simultaneously.

Iso-electric focusing (IEF) has proven to be a very versatile separation method for the characterisation, identification, purity determination, and quantitation of amphoteric substances such as peptides and proteins.