2011年11月1日 · iCIEF is a more suitable technique to assess charge heterogeneities of ADCs. The separation mechanism of this electrophoretic technique is based primarily on pI differences of protein charge variants.

icIEF is a technique to separate proteins based on their isoelectric point. See how Maurice an automated icIEF instrument can simplify protein charge analysis.

2022年5月1日 · Capillary isoelectric focusing with whole-column imaging detection (icIEF) technology is now the mainstream technology to perform analytical isoelectric focusing analysis in pharmaceutical industry.

Having been amply described already in 1960-1961, how comes that isoelectric focusing (IEF) went largely unnoticed for just about a decade? Well Harry was a free-lance scientist in a …

Isoelectric focusing (IEF), also known as electrofocusing, is a technique for separating different charged molecules by differences in their isoelectric point (pI). [1][2] It is a type of zone electrophoresis usually performed on proteins in a gel that takes advantage of the fact that overall charge on the molecule of interest is a function of t...

Experience icIEF separation and UV detection coupled with mass spectrometry identification on the ZenoTOF 7600 system. One workflow, on a single platform, enables rapid monitoring of intact biotherapeutics and identification of charge variants throughout the drug development pipeline.

2023年2月5日 · Since the first commercial imaged capillary isoelectric focusing (icIEF) instrument was developed twenty years ago, the technology has become the gold standard of quality and manufacturing process control in the biopharmaceutical industry.

2025年3月1日 · We present an integrated protocol for studying the charge heterogeneity of therapeutic proteins combining two workflows: imaged capillary isoelectric focusing (icIEF) with fractionation and online mass spectrometry (MS) detection on a single platform.

To support analysis of the charge heterogeneity of USP mAbs, we developed methods for cIEF using the SCIEX platform (PA 800 Plus) and icIEF using Bio-Techne’s Protein Simple Maurice and iCE3 platforms (Table 3).

Imaged capillary isoelectric focusing (icIEF) has made remarkable strides in recent years, presenting the biopharmaceutical industry with a range of innovative solutions to characterize protein charge heterogeneity and streamline the development and manufacturing of biologics.