2012年10月19日 · The unraveling of the mechanism of plasmid stabilization yielded a surprise: hok / sok increases plasmid maintenance by killing of plasmid-free cells, a mechanism known as post-segregational killing (PSK). The hok / sok locus codes for two RNAs: Sok antisense RNA that inhibits translation of the toxin-encoding hok mRNA.

We revealed the death-inducing phenotype of the Hok toxin when it is expressed from the chromosome in the absence of the antitoxin and recovered previously described intragenic toxicity determinants of this system. We identified nucleotides that are essential for the transcription, translation or activity of Hok.

在这项工作中，我们研究了具有诱导型 T7lac启动子和卡那霉素抗性基因的标准pET28a质粒中hok / sok 元件的位置和方向的影响。 研究发现，无论 hok / sok 元件在质粒中的位置和方向如何，它都保留其功能活性。 在不使用抗生素的情况下培养四天后，细菌细胞保留了含有 hok / sok的质粒，而不含该元件的对照质粒则丢失了。 使用三种目标蛋白—— 大肠杆菌 II 型天冬酰胺酶 (ASN)、人生长激素 (HGH) 和 SARS-CoV-2 病毒核蛋白 (NP)——结果表明，细菌对细胞质蛋 …

1992年1月5日 · Hok mRNA encodes the Hok killer protein of 52 amino acid residues. Expression of hok is regulated post-transcriptionally by Sok antisense RNA.

2015年2月1日 · The hok / sok locus is a well established type 2 toxin/antitoxin system which was originally discovered due to its stabilizing activity on the inheritance of plasmid R1 [1]. It encodes a highly potent host killing (Hok) toxin, and an antisense RNA antitoxin, suppression of killing (Sok).

1992年1月5日 · The hok/sok locus of plasmid R1, which mediates plasmid stabilization by killing of plasmid-free segregants, codes for two RNAs, Hok mRNA and Sok antisense RNA. Hok mRNA encodes the Hok killer protein of 52 amino acid residues. Expression of hok is regulated post-transcriptionally by Sok antisense RNA.

The hok/sok locus is renowned for its plasmid stabilization effect via post-segregational killing of plasmid-free daughter cells. However, the function (s) of the chromosome-encoded loci, which are more abundant in pathogenic strains of a broad range of …

质粒R1的hok / sok基因座通过杀死无质粒的分离子介导质粒的稳定，编码两个RNA，即Hok mRNA和Sok反义RNA。 Hok mRNA编码52个氨基酸残基的Hok杀手蛋白。 hok的表达受Sok反义RNA转录后的调控。 hok / sok系统通过Hok和Sok-RNA的不同衰减来杀死无质粒子细胞：Hok mRNA非常稳定，而Sok-RNA迅速衰减，从而导致质粒中Hok mRNA翻译的抑制。 游离分离剂，确保快速选择性地杀死这些细胞。 Sok反义RNA与Hok mRNA的前导区互补。 但是，互补区域 …

In this work, we studied the effects of position and orientation of the hok / sok element in the standard pET28a plasmid with the inducible T7lac promoter and kanamycin resistance gene. It was found that the hok / sok element retained its functional activity regardless of its location and orientation in the plasmid.

2024年5月9日 · 迄今为止，hok/sok基因的研究多集中在探究其机制及其在合成生物学中的应用，但关于运用于提升蛋白水平的质粒稳定性系统鲜有报道，且hok/sok基因的插入位点的选择也会影响质粒的稳定性，且利用hok/sok基因提升质粒的稳定性仍是需要本领域技术人员进行探索的。 技术实现思路. 1、为了解决上述问题，本发明的目的是提供一种含hok/sok基因的菌株及其制备与应用。 hok/sok基因的应用范围更加广泛，不受限于发酵宿主与发酵条件。 与现有技术相比，本 …