G-Rex® stands for Gas Permeable Rapid Expansion and is the result of applying our disruptive technology to the needs of immunotherapy. G-Rex® is the only production platform created specifically to produce immune cells such as T cells, natural killer cells, and hematopoietic cells.

In addition, TIL grown in GRex plates showed a higher percentage of CD8+ cells (94±8% versus 76±14%, p=<0.001) and lower percentage of NK cells (2.4±3% versus 12±9%, p=<0.001) than those grown in polystyrene. Tumor‐ specific activity was comparable, as measured by IFN‐ γ secretion between the two culture conditions.

2022年1月19日 · Provides details on using G-Rex for an efficient approach to generate highly functional Vγ9Vδ2 T cells in massive numbers suitable for clinical application in an allogeneic setting. Comparison of TIL production in traditional method (Flask + gas permeable bag) vs G-Rex at MD Anderson.

常用于细胞免疫治疗的细胞包括： CAR-T 、 TCR-T 、 TIL 、 NK 、 CIK 等。 然而，与淋巴细胞的分离、修饰、扩增相关的技术和安全问题是限制免疫细胞治疗的重要因素。 细胞免疫治疗对于免疫活性细胞数量的要求极高。 以细胞因子诱导的杀伤细胞（CIK）为例，据报道，CIK细胞治疗的剂量是每次注射108-1010个细胞。 使用传统的细胞培养瓶扩增细胞，需要通过频繁操作来调整细胞浓度以及为细胞提供新鲜培养基，大大增加了污染的风险。 使用透气性培养袋或生物反应 …

Tumor-infiltrating lymphocytes (TIL) engineered with regulatable membrane-bound IL15 (mbIL15) and LIGHT (TNFSF14) show enhanced efficacy in fibroblast-containing cold tumors © 2024 Obsidian Therapeutics, Inc.

Adoptive cell transfer (ACT) with tumor-infiltrating lymphocytes (TILs) can generate durable clinical responses in patients with metastatic melanoma and ongoing trials are evaluating efficacy in other advanced solid tumors.

首先在G-Rex10中培养从肿瘤消化液和肿瘤碎片得到的TIL细胞，接下来使用能容纳500ml培养基的G-REX100进行快速的扩增培养。 来源于14位患者中13个的肿瘤消化液和全部11个肿痛碎片的TIL细胞，在G-Rex10中成功完成了初始生长。

Adoptive cell therapy (ACT) of metastatic melanoma with autologous tumor infiltrating lymphocytes (TIL) is clinically effective, but TIL production can be challenging. Here we describe a simplified method for initial TIL culture and rapid expansion in gas-permeable flasks.

“The Cell Processing Lab has shown that the culture of Tumor Infiltrating Lymphocytes (TIL), Natural Killer (NK) cells and LCL cells when using the G-Rex flasks are superior to growth bags. Gin -Rex flasks allow for cell growth at higher densities and therefore less media, growth factors, cytokine and serum additives are required. The NIH

G-Rex® is the only cell culture bioreactor equipped to meet current patient demand for immune cell therapies. Designed for practical cell expansion, G-Rex reproducibly generates a healthy, cell product while enabling parallel patient processing in an footprint.