Describes the procedure for the administration of fluids directly into the lower esophagus or stomach through the use of a standard gavage needle (or feeding needle) introduced into the mouth and threaded down the esophagus. With proper training, this method is performed easily and quickly with minimal distress to the animal.

Mouse: 18-20 gauge feeding tubes about 1.5 inches in length with a rounded/bulb tip (see picture below). If gavage is performed on young mice a smaller tube is used. Rat: 16-18 gauge feeding tube about 2-3 inches in length. • For large mice and small rats (30+ grams) an 18-gauge rounded/bulb tipped gavage needle can be used.

Flexible plastic gavage needles (mice or rats) or red rubber feeding tubes (for rats) have less chance of damaging the esophagus than stainless steel gavage needles, but animals may bite through them and

This study examines existing IACUC protocols for the oral administration via an oral–esophageal–gastric cannula (gavage) of mice which were proposed decades ago (before the ‘microbiome era’ started in the 2000s) to standardize the administration of liquid chemical substances to laboratory mice.

Gavage is used to administer precise amounts of liquid diet, drugs or test compounds by mouth directly to the stomach of the mice. To minimize stress to the animal, the person performing gavage must be skilled before starting the study.

Material can be administered orally using a number of different techniques, although gavage using a stomach tube is the most widely used. To minimise the risk of adverse effects associated with this procedure, it is important that the operator is skilled both in the technique and the restraint method needed.

研究中对10只雄性Wistar大鼠（体重220~250 g）和10只雄性CB17-SCID小鼠（体重24~28 g）进行插管和肺泡灌洗液收集。 结果共完成20次大、小鼠的插管和肺泡灌洗操作，成功收集到1×107个大鼠肺泡巨噬细胞和3× 106个小鼠肺泡巨噬细胞。 结论应用小鼠灌胃针和电泳点样吸头完成的收集大、小鼠肺泡灌洗液新方法，能够被广泛用于大、小鼠呼吸系统疾病模型的研究。

2024年6月1日 · In this study, we sought to validate the method of gastric gavage sampling of mice, demonstrating that the same mouse can be used and sampled repeatedly without disturbing the microbiota being sampled.

Introduction and Definitions: To administer precise amounts of liquid diet, drugs or test compounds orally to mice using the gavage method. To minimize stress to the animal, the person must be skilled in the gavage technique before starting a study.

Extended use of intragastric gavage results in a small amount of systemic antigen exposure. Mice were divided into 4 treatment groups (n = 3 per group): nongavage, syringe-fed water; nongavage, syringe-fed ovalbumin; gavage-fed water; and gavage-fed ovalbumin. Mice were received water or 3 mg ovalbumin in water every day for 14 d.