Fluo-4 is an analog of fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels.

Fluo-4 is used to measure calcium (Ca 2+) concentrations inside living cells, and is often used for high-throughput screening of receptor ligands and calcium permeable ion channels. The green-fluorescent calcium indicator, Fluo-4, is an improved version of the calcium indicator, Fluo-3.

Fluo-4 AM是最常用的检测细胞内钙离子浓度的荧光探针之一。 分子式为C 51 H 50 F 2 N 2 O 23，分子量为1096.95。 Fluo-4 AM在Fluo-3 AM的基础上改进而成，其特点是加载更快并且在相同条件下荧光更加明亮。 Fluo-4 AM将Fluo-3 AM结构中的氯原子替换成了氟原子，导致它的最大激发波长会向短波长处偏离12nm左右。 这个波长更接近于氩激光器的波长488nm，所以用氩激 …

The Fluo-4 Calcium Imaging Kit contains all of the necessary components for the detection of calcium flux by imaging applications. In addition to Fluo-4 dye, the kit contains PowerLoad™ Concentrate, a 10X Neuro Background Suppressor solution, and probenecid.

BD Pharmingen™ Fluo-4 AM is a cell-permeant dye that can be used to measure the intracellular ionized calcium concentration, [Ca 2+]i. The hydrophobic acetomethoxy (AM) moiety allows passage across the cell membrane into viable cells.

Fluo-4 AM is a cell-permeable Ca2+ (calcium ion) indicator. - Mechanism of Action & Protocol.

Fluo-4 AM is a widely used fluorescent dye for measuring intracellular calcium (Ca 2+) levels, a critical parameter in various physiological and pathological processes. The mechanism of Fluo-4 AM's operation involves several key steps, making it …

Fluo-4 AM is a cell-permeable calcium ion indicator that is metabolized by intracellular esterase, leading to a bright green fluorescent signal upon calcium ion binding. Fluo-4 AM is well suited for fluorometric and imaging applications.

2024年9月5日 · Fluo-4 AM is a cell permeable, green fluorescent calcium probe used in fluorescence microscopy and flow cytometry. Fluo-4 belongs to a class of intensiometric biosensor that detect calcium ions. It is a structural analog of Fluo-3, an earlier Ca 2+ probe, wherein chlorine has been substituted with fluorine.