In the Flp-In System, three different vectors are used to generate isogenic stable mammalian cells lines expressing your gene (s) of interest. The first major component of the Flp-In™ System is the pFRT/lacZeo target site vector that is used to generate a Flp-In host cell line.

Flp-In™系统使您可以稳定整合和表达感兴趣的基因，以提供单拷贝等基因细胞系。 Flp-In™表达会在您选择的哺乳动物细胞系基因组中引入一个Flp重组靶标（FRT）位点。

Flp-In™ T-REx™ 系统利用酿酒酵母来源 DNA 重组系统，简化了诱导型哺乳动物稳定表达细胞系的生成过程。 该 DNA 重组系统使用重组酶 (Flp) 和位点特异性重组（Craig，1988；Sauer，1994），有助于将目标基因整合至哺乳动物细胞基因组中的特定位点上。 pFRT/lacZeo 靶标位点载体：pFRT/lacZeo 载体包含 lacZ-Zeocin™ 融合基因，其表达受到 SV40 早期启动子的控制。 已插入的 FRT 位点刚好位于 lacZ-Zeocin™ 融合基因 ATG 起始密码 …

The Flp-In™ System allows stable integration and expression of your gene of interest to deliver single-copy isogenic cell lines. Flp-In™ expression involves introduction of a Flp Recombination Target (FRT) site into the genome of the mam

Flp-In Cell Lines are designed for rapid generation of stable cell lines that express a protein of interest from a Flp-In expression vector. These cells contain a single stably integrated FRT site at a transcriptionally active genomic l

Flp-In™ 293 cell line is designed for rapid generation of stable cell lines that ensure high level expression of your protein of interest from a Flp-In™ expression vector. These cells contain a single stably integrated FRT site at a transcriptionally active genomic locus.

2023年10月16日 · 本手册介绍了如何使用Flp-In™系统生成稳定的哺乳动物表达细胞系。 该系统使用Flp重组酶介导的整合技术。 系统包括Core System和Complete System。

2017年3月16日 · Flp-In™系统是将目的基因插入到哺乳动物细胞基因组中特定位置的一项基因表达技术。 利用Flp-In™系统构建重组细胞株需要两步：首先需要将Flp Recombination Target (FRT) 位点导入到宿主细胞基因组中；然后通过Flp重组酶介导的DNA重组将目的基因插入到FLP位点。

The Flp-In™ cell lines stably express the lacZ-Zeocin™ fusion gene and are designed for use with the Flp-In™ System (Cat. nos. K6010-01 and K6010-02). Each cell line contains a single integrated Flp Recombination Target (FRT) site from pFRT/lacZeo or pFRT/lacZeo2 as confirmed by Southern blot analysis. See “Parental cell lines“ on

In the Flp-In™ System, three different vectors are used to generate isogenic stable mammalian cells lines expressing your gene(s) of interest. The first major component of the Flp-In™ System is the pFRT/lacZeo target site vector that is used to generate a Flp-In™ host cell line. The vector contains a lacZ-Zeocin™ fusion gene