In this study, a system involving the synthesis pathway and regeneration of FAD was engineered in Escherichia coli to improve α-keto acid production-from the corresponding l-amino acids-catalyzed by FAD-dependent l-amino acid deaminase (l-AAD).

2022年1月24日 · Here we report the identification of a promiscuous flavin-dependent nitroreductase, BaNTR1, that is able to promote enantioselective photobiocatalytic reductions of a broad range of structurally diverse ketones to yield the corresponding alcohols with high conversion (up to >99 %) and outstanding enantiopurity (up to >99 : 1 e.r).

In this study, a system involving the synthesis pathway and regeneration of FAD was engineered in Escherichia coli to improve α-keto acid production-from the corresponding l-amino acids-catalyzed by FAD-dependent l-amino acid deaminase (l-AAD). First, key genes, ribH, ribC, and ribF, were overexpressed and fine-tuned for FAD synthesis.

2019年3月4日 · Flavin Oxidoreductase-Catalyzed Regeneration of NAD + for the Oxidation of CDCA. FRs catalyze the reduction of free flavins (riboflavin, FMN, or FAD) by using reduced pyridine nucleotides, NADPH, or NADH.

2020年1月1日 · Flavin-dependent enzymes utilize flavin derivatives derived from vitamin B2 or riboflavin as cofactors or co-substrates to catalyze their reactions. These enzymes can catalyze a wide range of catalytic reactions including redox and non-redox types.

facile oxidation of aldehydes and α‐keto acids by flavin as catalyzed by thiazolium ion and cationic micelle. Chemischer Informationsdienst 1981 , 12 (14) https://doi.org/10.1002/chin.198114340

2022年2月19日 · Here we report the identi-fication of a promiscuous flavin-dependent nitroreductase, BaNTR1, that is able to promote enantioselective photobiocata-lytic reductions of a broad range of structurally diverse ketones to yield the corresponding alcohols with high conversion (up to. > and outstanding enantiopurity (up to >99:1 e.r).

2023年12月29日 · Flavin adenine dinucleotide (FAD) is a universal cellular cofactor involved in biological redox and radical metabolism reactions. FAD biosynthesis from riboflavin typically proceeds through two ATP-dependent enzymatic reactions, with flavin mononucleotide (FMN) as the intermediate.

Herein, we report the photocatalytic oxyfunctionalization of alkynes with electron-withdrawing groups to afford α-keto ketals with alcohols and molecular oxygen using riboflavin tetraacetate (RFT) as a powerful photoredox catalyst by binding to two scandium ions ( [RFT-2Sc 3+]).

2017年5月12日 · In this study, a system involving the synthesis pathway and regeneration of FAD was engineered in Escherichia coli to improve α‐keto acid production—from the corresponding l‐amino acids—catalyzed by FAD‐dependent l‐amino acid deaminase (l‐AAD). First, key genes, ribH, ribC, and ribF, were overexpressed and fine‐tuned for FAD synthesis.