FLP-FRT recombination - Wikipedia
In genetics, Flp-FRT recombination is a site-directed recombination technology, increasingly used to manipulate an organism's DNA under controlled conditions in vivo.
基因条件性敲除与Cre/LoxP、Flp/FRT重组系统 - 知乎
利用Cre/LoxP和来自酵母的Flp/FRT系统可以研究特定组织器官或特定细胞中靶基因灭活所导致的表型。 通过常规基因打靶在基因组的靶位点上装上两个同向排列的loxp，并以此两侧装接 …
FLP-FRT系统--诱导性基因编辑inducible gene editing - 简书
2019年2月13日 · FLP是一个重组酶（flippase，Flp），类似于Cre，它识别的位点是FRT，当FRT在同一条DNA上且方向相同时，则两FRT位点之间的基因则被删除；FRT方向相反，则基 …
The Cre-lox and FLP-FRT systems - The Jackson Laboratory
2006年5月19日 · Two of the most exciting and versatile genetic tools designed in the last 30 years are the Cre-lox and FLP-FRT technologies. Both allow the location and timing of gene …
Cre/loxP, Flp/FRT Systems and Pluripotent Stem Cell Lines
2012年1月1日 · Cre and FLP recombinases are the most widely used site-specific recombinases in genome engineering. Both are members of the tyrosine class of recombinases and catalyze …
Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View …
Cre-mediated possibilities for site specific (and often cell type specific) control of DNA recombination and gene expression can be advanced by the coordinated use of fellow SSR …
A “FLP-Out” System for Controlled Gene Expression in …
We present a two-part system for conditional FLP-out of FRT-flanked sequences in Caenorhabditis elegans to control gene activity in a spatially and/or temporally regulated …
Flp-In系统：构建稳定表达细胞系-赛默飞| Thermo ... - CN
In the Flp-In System, three different vectors are used to generate isogenic stable mammalian cells lines expressing your gene (s) of interest. The first major component of the Flp-In™ System is …
Genome targeting by hybrid Flp-TAL recombinases
2020年10月15日 · Flp-TAL recombinases are hybrid enzymes that are composed of two functional modules: a variant of site-specific tyrosine recombinase Flp, which can have either …
Application of the FLP/LoxP-FRT recombination system to switch …
In this study, the FLP/LoxP-FRT recombination system was used as a gene switch, controlling the temporal and spatial specificity to precisely regulate target gene expression. The FLP …