E3 ligases are critical for modulating cellular homeostasis owning to their efficient regulation and substrate specificity during the cascade of ubiquitination.

2021年7月30日 · E3 ubiquitin ligases are a large family of enzymes that join in a three-enzyme ubiquitination cascade together with ubiquitin activating enzyme E1 and ubiquitin conjugating …

The E3 ligases of the HECT (homologous to the E6AP carboxyl terminus) domain family catalyze ubiquitin transfer to the substrate protein through a two-step reaction: ubiquitin is first …

2017年6月20日 · Ubiquitin E3 ligases control every aspect of eukaryotic biology by promoting protein ubiquitination and degradation. At the end of a three-enzyme cascade, ubiquitin …

2023年11月23日 · We show that BioE3 identifies both known and new targets of two RING-type E3 ligases: RNF4 (DNA damage response, PML bodies), and MIB1 (endocytosis, autophagy, …

2014年4月4日 · Three distinct classes of E3 ligases have been identified that stimulate transfer of ubiquitin and ubiquitin-like proteins through either a direct or an indirect mechanism. Only...

2016年8月3日 · Ubiquitin ligases (E3s) recruit ubiquitin-conjugating enzyme (E2) ∼ ubiquitin (∼ denotes a thioester linkage; here, the linkage is between the catalytic cysteine of E2 and the …

The selectivity of the ubiquitin-26 S proteasome system (UPS) for a particular substrate protein relies on the interaction between a ubiquitin-conjugating enzyme (E2, of which a cell contains …

2024年12月17日 · Recruitment by an E3 ligase is a mandatory step in PROTACs and molecular glues mechanism of action. E3 ligases have diverse temporal, spatial and disease-related …

2021年9月2日 · Here we review the existing E3 ligases that have thus far been successfully exploited for TPD and discuss chemoproteomics-enabled covalent screening strategies for …