e-values in blast results represent the probability of the alignment occurring by chance. It is a statistical calculation based on the quality of alignment (the score) and the size of the …

2006年11月6日 · I'm trying to parse Blast results in order to sort them (according to relevant parameters of e-value, length of alignment, ...) as to retrieve only good alignements of cDNAs …

2009年8月11日 · The value of BLASTing your primers is diminished by the fact that even if BLASTing shows a good match -- say 20 of 21 bases -- if the 3' base is wrong, the primer will …

STANARD DEVIATION and t-value - (reply: 1) 394. Vector NTI's local database - (reply: 2) 395. four questions about using BLAST search - (reply: 1) 396. question from a non-bioinformatics …

Primer Design with Primer-Blast - My experience has been worse than just using Primer3 and BLAT separate (Oct/19/2010 ) I'm trying to design primers for the following sequence. The …

Interpreting primer BLAST scores for self-complementary - (Jul/18/2014 ) I can't find this info anywhere. I designed a primer pair w/IDT tools and they came out fine for homo- and …

2007年4月30日 · i. e. DMEM (w/ sodium pyruvate, glucose, sodium bicarbonate and L-glutamine) DMEM (w/ sodium pyruvate, glucose, sodium bicarbonate; w/o phemol red and L-glutamine) …

You can also use Primer-BLAST, which is quite easy. Put your template sequence, then add the sequences of your for and rev primers (5'->3', as you ordered them) and it will give you a nice …

2005年6月8日 · in the flat file u can take also same bp as in the blast hit the sbjct is shown from where it has 100% similarity .so u take some 1500 bp before that and some base pairs after …

The primer-template Tm is a real experimentally observable event, that depends on many things, on concentration of the primers and template, amount of salts, dNTPs and so on.