
活体成像中荧光染料的选择与成像 - 知乎 - 知乎专栏
Cy5.5 (Ex/Em:678/701 nm)和 Cy7 (Ex/Em:749/776 nm)是对分子标记的最优选择之一; DiD (Ex/Em:644/663 nm)、 DiR (Ex/Em:748/780)染料则常用于活体成像实验中对细 …
小动物活体成像 - 知乎 - 知乎专栏
IVIS成像系统的荧光成像波长范围为400-950 nm,针对相当宽范围的 荧光染料 和 荧光蛋白 的应 用。 内置各种市面上流通的商品化荧光剂(DID,DIR,CY5.5……),也可以定制荧光波长。
IVIS 活体光学成像技术概述-上海玮驰仪器有限公司
2024年5月13日 · IVIS Spectrum 独有的 3D 成像功能可以对荧光标记的抗肿瘤药物在脑胶质瘤内的靶向性进行成像和精确 定位,从而为研究肿瘤微环境对药物释放、药效和药物靶向作用提供 …
In vivo tracking of bioorthogonally labeled T-cells for predicting ...
2021年1月10日 · The in vivo biodistribution and tumor accumulation of Cy5.5-CTLs were non-invasively tracked for 7 days by using NIRF imaging system (IVIS-Lumina III In Vivo Imaging …
In Vivo Spinal Distribution of Cy5.5 Fluorescent Dye after Injection ...
In this study, we optimized surgical procedures for CM and LV injection of Cy5.5 into CSF of rats and evaluated spatiotemporal distribution of Cy5.5 within CSF of rats. The CM includes three …
In vivo tracking of cubosomes by Cy5 fluorescence. (A) IVIS …
(A) IVIS images of whole organs, namely lung, heart, brain, tumor, liver, spleen, and kidney as numbered from 1 to 7 in the image, showing uptake of Cy5 in various organs in the Affimer...
结论:通过光谱分离技术, 大幅度提高了信号的信噪比,并能非常便捷地同时分离不同荧光染料信号;不同荧光染料信号可以单独呈现, 也可以Overlay 显示。 去除背景信号或干扰信号,提高定量的准 …
活体动物荧光成像技术原理及应用 - 丁香园
2012年2月6日 · 分子探针的一端联有能够和生物体内特异靶点结合的分子结构(如肽类、酶的底物、配体等),另一端则是荧光染料。通过Cy5.5标记的抗体的体内代谢实验,可见肝、肾等处 …
Biodistribution of Cy5-MGdNPs in mice imaged using a
We encapsulated gadolinium oxide (Gd2O3) nanoparticles within phospholipid micelles as a novel low cytotoxic T 1-weighted MRI imaging contrast agent (MGdNPs) that can also deliver small …
Active loading of cyanine 5.5 derivatives into ... - RSC Publishing
In vivo fluorescence imaging (IVIS) demonstrated the same fluorescence activation in tumor-bearing mice intratumorally injected with the liposome. We believe that the developed active …