Carbol fuchsin, carbol-fuchsin, carbolfuchsin, or Castellani's paint is a mixture of phenol and basic fuchsin that is used in bacterial staining procedures. It is commonly used in the staining of mycobacteria because it has an affinity for the mycolic acids found in their cell membranes.

2024年9月2日 · Carbol-Fuchsin is key for acid-fast bacteria stains (Ziehl-Neelsen, Kinyoun's, Fite) by resisting decolorization, detecting bacteria missed by Gram stains.

Carbol Fuchsin is a basic dye which forms a yellow-brown compound in presence of acid. It is mainly required for the staining of mycobacteria. The waxy walls of mycobacteria helps in retaining the dye within cells. It can also be used for the staining of bacterial spores. [1] Mixture of basic fuchsin and phenol. Danger.

Carbol fuchsin is more effective than acetocarmine or Giemsa stain for rose chromosomes. Carbol fuchsin-stained chromosomes darken with time.

Principle hence absorbs dyes only very slowly. The most eficient staining meth-od is the hot staining according to Ziehl-Neelsen. In this method, carbol-fuchsin solution i applied to the specimen and heated. This heating process accelerates the rate at which the fuchsin dye is absorbed and thus also that of the formation of the mycol

Ziehl-Neelsen carbolfuchsin solution - for microscopy, is a ready-to use solution used in the Ziehl-Neelsen hot staining for the microscopic investigation of acid-fast bacteria (AFB). The cell wall …

Ziehl-Neelsen staining method using Carbol-Fuchsin is a crucial diagnostic tool used for the detection of acid-fast mycobacteria in tissue sections. Carbol-Fuchsin solution according to …

La carbol fucsina se utiliza como tinte principal para detectar bacterias acidorresistentes porque es más soluble en las células. lípidos de la pared que en el alcohol ácido. Si la bacteria es resistente al ácido, conservará el color rojo inicial del tinte porque puede resistir la decoloración del alcohol ácido (0,4-1% HCl en 70% EtOH).

En la tinción de Ziehl-Neelsen se utiliza el compuesto fenólico carbol fucsina, un colorante básico. Este tiene la capacidad de interactuar con los ácidos grasos de la pared celular, la cual es de textura cerosa a temperatura ambiente.