New function of a well-known promoter: enhancer activity of minimal CMV ...
The regulatory function of a core fragment (cC) from human cytomegalovirus (CMV) promoter was investigated with cell-lineage specificity in NIH3T3 (fibroblast) and hematopoietic cell lines …
Highly efficient ex vivo lentiviral transduction of primary human ...
2019年11月1日 · We show that protamine sulfate-assisted transduction of a VSV-G-pseudotyped vector expressing eGFP under the control of a CMV promoter in a serum-free environment …
New function of a well-known promoter: Enhancer activity of minimal CMV ...
Results: The cC element in reverse-orientation not only boosted upstream Es promoter to levels comparable to full-length EF in DAMI, U937 and 3T3 cells, but also blocked the suppression of …
Lentiviral Vectors Efficiently Transduce Quiescent Mature 3T3-L1 ...
2004年2月1日 · In this report, we evaluated the use of lentivirus-mediated gene transfer into 3T3-L1 mature adipocytes. We demonstrate that quiescent, fully differentiated 3T3-L1 adipocytes …
具有丰富 CXCR4 显示的细胞膜囊泡增强其作为药物载体向炎症部 …
本研究将CXCR4重组慢病毒转染MC-3T3细胞，获得膜上富含CXCR4的MC-3T3细胞。 从转染的 MC-3T3 细胞中获得具有富集膜 CXCR4 显示的 CMV（CXCR4-CMV）。 姜黄素是一种有效的 …
A bidirectional promoter architecture enhances lentiviral transgenesis ...
2011年3月10日 · In somatic NIH 3T3 cells, the γ-retroviral vector LMP and bidirectional CMV construct PCG(Bi) produced the strongest (60%) reduction in firefly luciferase activity as …
Cytomegalovirus Cell Tropism, Replication, and Gene Transfer in …
1999年12月12日 · We used recombinant murine cytomegalovirus (mCMV) expressing either green fluorescent protein (GFP) or β-galactosidase under control of human elongation factor 1 …
Enhancer Requirement for Murine Cytomegalovirus Growth …
As expected, numerous plaques developed in NIH 3T3 cells when transfected with the wild-type MCMV BAC plasmid, pSM3, and cultures reached a complete cytopathic effect within 9 days. …
Efficient adenovirus transduction of 3T3-L1 adipocytes stably ...
2003年3月7日 · We have developed and characterized a 3T3-L1 adipocyte cell line stably expressing the truncated receptor for coxsackievirus and adenovirus receptor (CAR) for its …
LTR–miRNA–Puro (LMP) and lentiviral CMV GFP-IRES-neomycin (CMV GIN) retroviral vectors, and as a negative control with bidirectional CMV and EEF1A1 lentiviral constructs expressing …