CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 promotes T cell activation through its association with the TCR complex and protein tyrosine kinase lck.

CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation and has been shown to play a role in thymic differentiation.

The CD8 antibody, clone SK1, is derived from hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human peripheral blood T lymphocytes. The CD8 antibody recognizes the 32-kilodalton (kDa) α-subunit of a disulfide-linked bimolecular complex.

CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells. Applications Reported: The 53-6.7 antibody has been reported for use in flow cytometric analysis.

Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation, and has been shown to play a role in thymic differentiation.

Invitrogen Anti-CD8 alpha Monoclonal (KT15), Catalog # MA5-16759. Tested in Flow Cytometry (Flow) applications. This antibody reacts with Mouse samples. Supplied as 100 µg purified antibody (0.1 mg/mL).

Human peripheral blood lymphocytes were stained using FITC of anti-human CD8 monoclonal antibody (clone 3B5). The negative control profiles represent unstained cells. Commonly used, FITC conjugates provide relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility.

The G42-8 monoclonal antibody specifically recognizes the alpha subunit (32 kDa) of CD8αα homodimer or CD8αβ heterodimer. The CD8 molecule binds to HLA class I molecules during interaction of CD8+ T cells with antigen-presenting cells or target cells. CD8 is expressed on T cytotoxic/suppressor cell populations.

Anti-human CD8, clone UCHT-4, is a monoclonal antibody designed to be used as a direct immunofluorescence reagent in order to identify cells that express the CD8 antigen by flow cytometry. Fluorescein (FITC) is part of the BD blue family of dyes.