The bicinchoninic acid assay (BCA assay), also known as the Smith assay, after its inventor, Paul K. Smith at the Pierce Chemical Company, [1] now part of Thermo Fisher Scientific, is a biochemical assay for determining the total concentration of protein in a solution (0.5 μg/mL to 1.5 mg/mL), similar to Lowry protein assay, Bradford protein ...

Features of the BCA Protein Assay Kit include: How the assay works. The BCA Protein Assay combines the well-known reduction of Cu 2+ to Cu 1+ by protein in an alkaline medium with the highly sensitive and selective colorimetric detection …

2016年10月27日 · BCA蛋白浓度测定试剂盒 (BCA Protein Assay Kit)是根据目前世界上最常用的两种蛋白浓度检测方法之一BCA法研制而成，实现了蛋白浓度测定的简单、高稳定性、高灵敏度和高兼容性。

二喹啉甲酸 (Bicinchoninic acid，BCA )法是 Lowry测定法 的一种改进方法，是近来广为应用的蛋白定量方法。 其原理与Lowery法蛋白定量相似，即在碱性条件下蛋白质分子中的肽键能与Cu2+络合生成络合物，同时将Cu2+还原成Cu+。 二喹啉甲酸及其钠盐是一种溶于水的化合物，在碱性条件下，可以和Cu+结合形成稳定的紫蓝色复合物，在562 nm处有高的光吸收值，并且化合物颜色的深浅与蛋白质浓度成正比，据此可测定蛋白质浓度。 制备BSA标准样。 先配制1 ml BSA母 …

BCA 全称是Bicinchoninic Acid Assay， 也叫Smith Assay，因为这个方法是Paul K. Smith 发明的……这个方法通过展示不同浓度样品的颜色变化来判断蛋白的浓度。 BCA分析主要依靠两个反应。

Explore our BCA assay kits, that provide high-precision, detergent compatible protein quantification determination.

Pierce BCA 蛋白定量试剂通过在碱性环境中用蛋白质将 Cu 2+ 还原为 Cu + （广为人知的双缩脲反应），然后通过二辛可宁酸（BCA）对亚铜阳离子（Cu + ）进行高灵敏度和高选择性的比色检测。

The Pierce BCA Protein Assay is a detergent-compatible formulation based on bicinchoninic acid (BCA) for the colorimetric detection and quantitation of total protein.

2020年11月1日 · Amongst the available methodologies for protein determination, the bicinchoninic acid (BCA) assay highlights for its simplicity, sensitivity, repeatability and reproducibility.

The bicinchoninic acid (BCA) assay is one of many ways to measure the total protein concentration of an unknown sample. In this assay, copper (II) [Cu 2+] is reduced to copper (I) [Cu 1+] in the presence of peptides in alkaline solutions. BCA can chelate (bind to) Cu 1+, creating a purple color