2014年9月3日 · Here we use whole-genome resequencing of six 293 cell lines to study the dynamics of this aneuploid genome in response to the manipulations used to generate common 293 cell derivatives, such as...

2016年9月20日 · In this study, we present the first comprehensive analysis of the protein, messenger RNA and microRNA profiles of 293T cell-derived exosomes; then, we characterized these data using Gene Ontology annotation and Kyoto Encyclopedia for Genes and Genomes pathway analysis.

2022年8月1日 · Here, we analyzed transcriptome changes in HEK 293T cells after depletion of each DNA-PK subunit. Depletion of various DNA-PK subunits resulted in dramatic differences in the number of differentially expressed genes: only 7 genes changed more than 2-fold in DNA-PKcs-deficient cells, 29 genes in Ku80-deficient, 219 genes in Ku70-deficient.

2023年5月16日 · In this work, the mRNA expression profile of three HEK-293 cell lines, resulting in various yields during a fermentation batch process for rAAV production, was investigated to gain basic insight into cell variability and eventually …

2023年5月12日 · Here, we develop a droplet-based snRNA sequencing technology (snRandom-seq) for FFPE tissues by capturing full-length total RNAs with random primers. snRandom-seq shows a minor doublet rate (0.3%),...

Here we present transcriptomic analysis of HEK293T cells treated with control siRNA (293T + siCtr), HEK293T with monoallelic knockout of Ku70 [1,2] treated with siCtr (293TΔKu70 + siCtr) or siKu70 (293TΔKu70 + siKu70), HEK293T with monoallelic knockout of Ku80 treated with siCtr (293TΔKu80 + siCtr) or siKu80 (293TΔKu80 + siKu80), and ...

Compare the variations of 6 HEK293-cell-lines with each other and the reference sequence. The HEK293 (Human Embryonic Kidney 293) cell line is one of the most frequently used cell lines in cell biology, second only to HeLa cells.

Total RNA extracted from 293T cells and circ-plasmid transfected 293T cells were firstly incubated with 20 U RNase R at 37 °C for 30 min to digest linear RNA. Then, SNAs were added to the mixture and incubated at 37 °C for 2 h. The mixture was mounted on a glass slide and examined by CLSM using a 488 nm laser as the excitation light source.

SHENTEK® 293T 总RNA 残留检测试剂盒适用于定量检测生物制品中残留的293T细胞总RNA; 通过设计特异性引物和探针, 将反转录和荧光探针qPCR 检测技术融合,实现一步法定量检测总RNA 残留; 其检测快速, 专一性强, 性能可靠。 100 Reactions。 规定储存条件下24 个月, 具体详见试剂盒标签。 293T RNA 定量参考品浓度标注于管壁标签, 请确认后再进行稀释。 用试剂盒中提供的RNase-Free H2O 将293T RNA 定量参考品进行梯度稀释,具体操作如下:1. 将试剂盒中的293T …

2022年12月24日 · HEK 293T cells treated with LNPs containing mRNA were able to access nutrients and produce protein for more than 24 h implying that the inevitable release of pseudouridine upon mRNA degradation...