2024年11月13日 · Plasmid v5 ABE-eVLP from Dr. David Liu's lab contains the insert MMLVgag (C507V)–ABE8e and is published in Nat Biotechnol. 2024 Nov 13. doi: 10.1038/s41587-024-02467-x. This plasmid is available through Addgene.

2022年1月11日 · Methods to deliver gene editing agents in vivo as ribonucleoproteins could offer safety advantages over nucleic acid delivery approaches. We report the development and application of engineered DNA-free virus-like particles (eVLPs) that efficiently package and deliver base editor or Cas9 ribonucleoproteins.

2024年11月13日 · Engineered virus-like particles (eVLPs) are promising vehicles for transient delivery of proteins and RNAs, including gene editing agents. We report a system for the laboratory...

2024年1月8日 · Here we report prime editor engineered virus-like particles (PE-eVLPs) that deliver prime editor proteins, prime editing guide RNAs and nicking single guide RNAs as transient ribonucleoprotein...

2024年11月15日 · 衣壳突变组合提高eVLP效能 通过对36种衣壳突变体的筛选和组合优化，作者发现GagC507V–ABE与GagQ226P–Pro–Pol组合（v5 BE-eVLPs）显著提高了eVLP的基因编辑效能，比v4 BE-eVLPs提高了3.7倍。

By engineering VLPs to overcome cargo packaging, release, and localization bottlenecks, we developed fourth-generation eVLPs that mediate efficient base editing in several primary mouse and human cell types. Using different gly-coproteins in eVLPs alters their cellular tropism.

2024年11月，Aditya Raguram等研究人员在Nature Biotechnology上发表了题为“Directed evolution of engineered virus-like particles with improved production and transduction efficiencies”的研究论文。 研究团队此前开发的Engineered...

2022年1月22日 · 我们报告了工程化无DNA病毒样颗粒（engineered DNA-free virus-like particles，eVLPs）的开发和应用，这种工具能有效地包装和递送碱基编辑或Cas9核糖核蛋白。 为了克服基因编辑中包装、释放和定位的瓶颈，我们以VLPs为基础开发了第四代eVLPs，在几种主要的小鼠和人类细胞类型中成功介导了高效的碱基编辑。 在eVLPs中使用不同的糖蛋白会改变它们的细胞趋向性。 向小鼠一次注射eVLPs可以在多个组织中实现治疗水平的碱基编辑，在63%的 …

研究人员开发了一种针对工程病毒样颗粒 (eVLP）的定向进化系统，创建了具有增强的生产、转导和基因编辑效率的第五代eVLP，为改善基因传递载体在治疗中的应用铺平道路。

最近报道了腺嘌呤碱基编辑器 (ABE)的高效体内递送：单向导RNA (sgRNA)核糖核蛋白 (RNPs)与迭代工程病毒样颗粒 (eVLPs)克服了货物包装、释放和定位中的特定分子瓶颈。