2018年10月14日 · tdTomato is a basic (constitutively fluorescent) orange fluorescent protein published in 2004, derived from Discosoma sp.. It is reported to be a somewhat slowly-maturing tandem dimer with low acid sensitivity.

Tandem dimer Tomato (tdTomato) provides a useful alternative to enhanced green fluorescent protein (eGFP) for performing simultaneous detection of fluorescent protein in histological sections together with fluorescence immunohistochemistry (IHC). eGFP has many properties that make it useful for cell …

About tdTomato. tdTomato is a tandem dimer fluorescent protein derived from the coral anemones species Discosoma sp. It has an excitation peak at 554 nm and an emission peak at 581 nm.

2021年11月25日 · 通常被命名为 红色荧光蛋白 RFP的探针如 DsRed 、TagRFP及 tdTomato，实际上具有明显的橙色多于红色的发射谱。 不考虑颜色的指示，用标准的 四甲基罗丹明异硫氰酸脂 （TRITC）滤光片设备，橙色光谱型的蛋白在多种颜色，如蓝绿色、绿色和红色情景中更易于成像。 在所有光谱型中，亮度最强的荧光蛋白是串联形式的二聚体Tomato（dimeric Tomato, …

tdTomato is an exceptionally bright red fluorescent protein—6X brighter than EGFP. tdTomato’s emission wavelength (581 nm) and brightness make it ideal for live animal imaging studies. The tdTomato fluorescent protein is as photostable as mCherry (Shaner et al. 2004).

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures. SnapGene Viewer is free software that allows molecular biologists to create, …

Tandem-dimer tomato (tdTomato) is a stable, robust red fluorescent protein that is nearly threefold brighter than GFP. Our objective was to create a line of transgenic rats that ubiquitously express tdTomato in all cells, driven by the human ubiquitin C promoter.

2010年12月1日 · Tandem dimer Tomato (tdTomato) provides a useful alternative to enhanced green fluorescent protein (eGFP) for performing simultaneous detection of fluorescent protein in histological...

2024年6月1日 · Fluorescent proteins have been widely used to trace biological events in animal models. TdTomato expression led to unexpected retinal dysfunction and ultrastructural defects in the transgenic mouse retina. Retinal dysfunction was seen as the reduced photopic responses and lower contrast sensitivity.

tdTomato is a red fluorescent protein with an excitation peak of 554nm and an emission peak of 581nm in vitro. It is an artificial derivative of the naturally occurring fluorescent protein encoded by the Discosoma drFP583 gene ( GenBank:AF168419 ; AAF03369), consisting of a tandem dimer of the dTomato fluorescent protein linked by a 12 residue ...