First, the assembly of a four-stranded antiparallel β-sheet between the β hairpins of nNOS and of PSD95-PDZ2, mainly enthalpic in nature, contributes 80% to the affinity. Second, binding is entropically reinforced by the hydrophobic interaction between side chains of the same nNOS β-hairpin with the side chains of α2-helix at the binding ...

Here, we present the solution structure of the nNOS PDZ domain complexed with a seven-residue target peptide. The three-dimensional structure of the complex provides insights into the structural...

2018年8月14日 · PSD95 is one of the regulatory proteins that interact directly with the PDZ domain of nNOS 52. In the present study, we first investigated if PSD95/nNOS interaction is induced by fear...

2023年4月23日 · 神经元型NOS（nNOS）的结构特征有别于其同工酶诱生型NOS（iNOS）和内皮型NOS（eNOS），其氮端含有PDZ结构域，介导多种类型的蛋白-蛋白相互作用，包括与突触后致密蛋白95（post-synaptic density 95, PSD-95）、nNOS羧基端PDZ配体蛋白（carboxy-terminal PDZ ligand of nNOS, CAPON）和5 ...

Here, we show that the N-terminus of nNOS, which contains a PDZ protein motif, interacts with similar motifs in postsynaptic density–95 protein (PSD-95) and a related novel protein, PSD-93. nNOS and PSD-95 are coexpressed in numerous neuronal populations, and a PSD-95/nNOS complex occurs in cerebellum.

2000年10月20日 · Here, we used PDZ domains from neuronal nitric oxide synthase (nNOS) and postsynaptic density protein-95 (PSD-95) to explore the mechanism for PDZ-dimer formation. The nNOS PDZ domain terminates with a ∼30 residue amino acid β-finger peptide that is shown to be required for nNOS/PSD-95 PDZ dimer formation.

在nNOS结构N末端具有一个PDZ结构域,可与含有PDZ结构域的蛋白相互作用。 nNOS在成熟和不成熟细胞中均有表达[5,6],主要集中于神经元细胞、中性粒细胞和星形胶质细胞中[7-9]。 同时nNOS也可存在于骨骼肌、心肌和平滑肌中,其产生的NO可控制血流和肌肉收缩。 nNOS可由不同的接头蛋白运输至不同的位置,这也可能导致其在不同部位具有不同的作用。 nNOS主要定位于质膜,近来有学者指出在一些神经元和神经胶质细胞中,未能发现nNOS定位于细胞质的情况下却发 …

1999年4月30日 · The nNOS-syntrophin PDZ complex structure revealed that the domains interact in an unusual linear head-to-tail arrangement. The nNOS PDZ domain has two opposite interaction surfaces—one face has the canonical peptide binding groove, whereas the other has a β-hairpin “finger.”

2020年12月10日 · Importantly, interactions of several proteins, including post-synaptic density 95 (PSD-95), carboxy-terminal PDZ ligand of nNOS (CAPON) and serotonin transporter (SERT), with the PSD/Disc-large/ZO-1 homologous (PDZ) domain of nNOS have been demonstrated to influence the subcellular distribution and activity of the enzyme in the brain.

1997年4月1日 · PDZ domains are multifunctional protein-interaction motifs that often bind to the C-terminus of protein targets. Nitric oxide (NO), an endogenous signaling molecule, plays critical roles in...