2019年3月27日 · The aim of this study was to focus the attention on non-active-site residues L209 and Y229 of NDM-1 and to investigate their role in the catalytic mechanism. Specifically, the …

In the L209F-Y229W enzyme, we observed a remarkable increase in kcat/Km of 370-, 140-, and 80-fold for cefepime, meropenem, and cefazolin, respec-tively. The same behavior was noted …

2013年12月10日 · Although residue Y229 is spatially distant from the active site and not contacting directly with the substrate or zinc ions, the Y229W mutant was found to have higher …

Although residue Y229 is spatially distant from the active site and not contacting directly with the substrate or zinc ions, the Y229W mutant was found to have higher kcat and Km values than …

2013年12月10日 · Although residue Y229 is spatially distant from the active site and not contacting directly with the substrate or zinc ions, the Y229W mutant was found to have higher …

这项研究的目的是将注意力集中在ndm-1的非活性位点残基l209和y229上，并研究它们在催化机理中的作用。 具体而言，通过抗菌药敏试验，动力学和分子动力学（MD）研究评估了L209F变 …

2012年1月23日 · The obtained models showed that both of the residues, Y229, located near the bottom of the α7 helix, and F603, located in the core of domain III, are involved in hydrophobic …

Although residue Y229 is spatially distant from the active site and not contacting directly with the substrate or zinc ions, the Y229W mutant was found to have higher kcat and Km values than …

The obtained models showed that both of the residues, Y229, located near the bottom of the α7 helix, and F603, located in the core of domain III, are involved in hydrophobic interactions …

2019年3月27日 · Specifically, the effect of the Y229W substitution in the L209F variant was evaluated by antimicrobial susceptibility testing, kinetic, and molecular dynamic (MD) studies. …