这项新的研究明确了XPA、XPB和XPD等蛋白的哪些部分参与了修复受损DNA的NER过程，以及它们的作用机制。 随着我们对XP患者中这些蛋白的致病变体所引起的结构变化对修复反应的负面影响的了解加深，也有可能在未来支持药物和其他治疗方法的开发。 1. Jinseok Kim et al. Lesion recognition by XPC, TFIIH and XPA in DNA excision repair.

xpa-xpg参与核苷酸切除修复（ner），而xpv编码参与前导链中受损dna复制的dna聚合酶。 NER和BER过程中的很多蛋白可以被DDR诱导，如p53、BRCA1可以上调DDB2、XPC、PCNA、FEN1等的表达。

2023年4月19日 · Here we report on structures revealing DNA lesion recognition by human XPC and DNA lesion hand-off from XPC to Core7 and XPA. XPA, which binds between XPB and XPD, kinks the DNA duplex and...

2020年2月28日 · Here we report cryo-electron microscopy structures of both DNA-free and DNA-bound human XPF-ERCC1. DNA-free XPF-ERCC1 adopts an auto-inhibited conformation in which the XPF helical domain...

In the following sections, we highlight current knowledge of the interactions of human XPA with DNA, other NER proteins and proteins outside of NER, and the relationship between XPA mutants and XP disorders. In the last section, we discuss future directions for XPA studies that can enrich our understanding of NER and XP disorders. 2.

Xeroderma pigmentosum complementation group A (XPA) is an essential protein in the nucleotide excision repair (NER) pathway, the main DNA repair pathway responsible for the excision of bulky DNA lesions in both eukaryotic and prokaryotic cells.

2020年2月7日 · Here, we show that TIP60, also known as KAT5, a haplo-insufficient tumor suppressor, directly acetylates XPF at Lys911 following UV irradiation or treatment with mitomycin C and that this...

2008年5月3日 · Two recent reports provide new physical information on how the XPA protein recruits the ERCC1-XPF heterodimer to the site of damage during the process of mammalian nucleotide excision repair (NER). Using chemical shift perturbation NMR experiments, the contact sites between a central fragment of ERCC1 and a XPA fragment have been mapped.

In this review, we focus on the interacting partners and mechanisms of regulation of the XPA protein. We summarize clinical oncology data related to this DNA repair factor, particularly its relationship with treatment outcome, and examine the potential of XPA as a target for small molecule inhibitors.

2012年8月24日 · Here we show that the NER factors XPG and XPF are essential for establishing CTCF-dependent chromatin looping between the promoter and terminator of the activated RAR β 2 gene. Silencing XPG and/or XPF endonucleases, or mutations in their catalytic sites, prevents CTCF recruitment, chromatin loop formation, and optimal transcription of RAR β 2.