Wings apart-like protein homolog (WAPL) is a protein that in humans is encoded by the WAPAL gene. [5] [6] [7] WAPL is a key regulator of the Cohesin complex which mediates sister chromatid cohesion, homologous recombination and DNA looping. Cohesin is formed of SMC3, SMC1, RAD21 and either SA1 or SA2.

2024年12月25日 · WAPL (WAPL Cohesin Release Factor) is a Protein Coding gene. Diseases associated with WAPL include Cornelia De Lange Syndrome and Mullegama-Klein-Martinez Syndrome. Among its related pathways are Separation of Sister …

2020年12月14日 · In this study, we used acute protein degradation to deplete WAPL in mouse embryonic stem cells (mESCs) to examine the immediate effects of changes in cohesin binding and 3D genome changes. We...

2013年8月25日 · In mitosis, Wapl-mediated release of cohesin from DNA is essential for proper chromosome segregation and protects cohesin from cleavage by the protease separase, thus enabling mitotic exit in the...

Mutations in the X-linked gene wings apart-like (wapl) result in late larval lethality associated with an unusual chromosome morphology. In brain cell metaphases of wapl mutants, sister chromatids of all chromosomes are aligned parallel to each other instead of assuming the typical morphology observed in wild type.

2020年7月1日 · Reduced Wapl expression causes global alterations in the chromosome architecture, indicating that the potential to recombine all V genes entails structural changes of the entire genome in pro-B...

近日，来自奥地利Vienna Biocenter的 Meinrad Busslinger 研究组在 Nature 上发表题为 Wapl repression by Pax5 promotes V gene recombination by Igh loop extrusion 的文章， 阐明Pax5通过抑制Wapl，从而实现对 lgh 的调控。 因为染色质环挤出依赖于黏连蛋白复合物，所以，作者首先研究在前体B细胞中，粘连蛋白复合物各组分的表达是否发生变化。 作者发现，在RNA水平上，相较于其他种类的免疫细胞，前体B细胞和原B细胞（pro-B cell）的的Wapl水平下调了四倍。 蛋白 …

WAPL cohesin 释放因子： 参与多个过程，包括 DNA 复制的负调控；染色质结合活性的负调节；和调节姐妹染色单体的凝聚力。 位于多种细胞成分中，包括高尔基体；细胞间桥；和微管细胞骨架。

Wapl depletion stabilizes Cohesin on chromatin, which causes interphase chromatin condensation as a consequence of Cohesin-mediated DNA loop extrusion (20, 42).

2024年1月7日 · Title: WAPL-Dependent Repair of Damaged DNA Replication Forks Underlies Oncogene-Induced Loss of Sister Chromatid Cohesion. Absolute copy numbers and dynamics of cohesin, CTCF, NIPBL, WAPL and sororin were measured by mass spectrometry, fluorescence-correlation spectroscopy and fluorescence recovery after photobleaching in HeLa cells.