What effect does vortexing have on a fluid sample that simple ...
The vortex not only provides a different profile of mixing, but has the power to mix viscous fluids that would otherwise remain virtually unmixed (glycerol stocks come to mind). Vortex shakers are contra-indicated for some lab protocols (like in miniprep kits) …
PCR Mastermix Preparation - Biology Stack Exchange
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What is the most critical step of plasmid purification?
2014年9月17日 · When mixing, it's only ok to vortex the cell pellet prior to lysis, after adding lysis buffer, ONLY MIX BY INVERTING THE TUBE. Vortexing after this point can shear DNA. If you overdry the pellets, you can resuspend them by leaving them in …
Why pellet and resuspend E. coli for plasmid prep
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Design primers for PCR from given DNA sequence
Stack Exchange Network. Stack Exchange network consists of 183 Q&A communities including Stack Overflow, the largest, most trusted online community for developers to learn, share their knowledge, and build their careers.
Why is DNA polymerase added at the end of PCR reaction?
PCR reaction is used to amplify DNA fragment. Each reaction requires a DNA template, buffer, dNTP mix and a unique pair of primers, one ''forward'' primer and one ''reverse'' primer. The DNA polyme...
How to prepare sample for SDS PAGE? - Biology Stack Exchange
2014年11月13日 · I usually take my protein sample 0.8ml and sample buffer(2X) 0.2ml for my sample preparation in SDS PAGE. Am I using correct proportion? my protein sample concentration is 4.1mg/ml.
Definition of "Effective population size" - Biology Stack Exchange
I'll add an informal answer to complement @Remi.b's excellent answer. In a very simple sense, you can think of the effective population size as the number of reproducing (breeding) individuals in a population.
Does mixing alcoholic drinks really make you more drunk?
There is plenty of anecdotal evidence ("beer after wine and you'll feel fine, wine after beer will make you feel queer") that mixing alcoholic drink types leads to a stronger effect, but I can't fi...
Why does absorption (at 260nm) of ssDNA increase with …
2020年1月6日 · In the graph below, I understand why A260 of double stranded DNA would increase with temperature increase, as more of the DNA becomes single stranded (and A260 of ssDNA is higher than that of dsDNA