How Influenza Virus Uses Host Cell Pathways during Uncoating
Our understanding of virus uncoating mechanisms has grown substantially in recent years and defines uncoating as a complex, highly orchestrated, multi-step process that relies on both viral and cellular factors. In this review, we describe the influenza A virus (IAV) infection cycle and focus on the capsid uncoating process.
Virus Uncoating - an overview | ScienceDirect Topics
Uncoating describes the events which occur after host cell entry, during which the virus capsid is partially or completely degraded or removed and the virus genome exposed, usually still in the form of a nucleic acid–protein complex. Uncoating occurs simultaneously with or immediately after entry and is thus difficult to study.
DNA virus uncoating - ScienceDirect
2015年5月1日 · During virus entry, capsid shells are primed and disassembled in a complex, tightly-regulated, multi-step process termed uncoating. Here we compare the uncoating-programs of DNA viruses of the pox-, herpes-, adeno-, polyoma-, and papillomavirus families.
Virus Replication - PMC
Uncoating refers to the breakdown or removal of the capsid, causing the release of the virus genome into the cell to wherever genome replication and transcription will take place. Uncoating can be separated from or tightly linked with penetration, and viruses achieve uncoating in a variety of different ways ( Fig. 4.5 ).
A quantitative model for virus uncoating predicts influenza A ...
Virus uncoating, the disassembly of the viral outer shell (capsid), is a particularly ill-characterized step because this transient phenomenon is hard to measure experimentally in vivo and to replicate in vitro. Studies in cells, including genetic and biochemical evidence, reveal uncoating instead of pre-programming as an active process relying ...
Principles of Virus Uncoating: Cues and the Snooker Ball
In this review, we discuss how cellular cues, facilitators, and built-in viral mechanisms promote uncoating. Cues come from receptors, enzymes and chemicals that act directly on the virus particle to alter its structure, trafficking and infectivity.
Mechanisms of virus uncoating - PubMed
In a virus particle, the genome is highly condensed and protected by proteins and membrane bilayers. Before it can be replicated in a new host cell, uncoating must take place. Recent studies on enveloped and nonenveloped animal viruses indicate that uncoating occurs through complex, multistep proces …
Unanchored ubiquitin in virus uncoating | Science - AAAS
2014年10月24日 · HDAC6 promotes viral uncoating, although the mechanism is not clear. Endosomal acidification not only triggers membrane fusion, but also activates the M2 ion channel. M2 pumps protons from the endosome into the virion, creating an acidic bath that debilitates M1 interaction with the viral RNP.
Influenza virus uses transportin 1 for vRNP debundling during cell ...
2019年1月28日 · Thus, IAV uncoating involves three major steps: priming, M1 shell disassembly and vRNP debundling. We have previously shown that histone deacetylase 6 (HDAC6) serves as a key cellular factor in...
Real-time dissection of dynamic uncoating of individual ... - PNAS
2019年1月9日 · The uncoating of influenza A virus (IAV), which contains an unusual genome of eight segmented RNAs, is particularly poorly understood. Here, by encapsulating quantum dot (QD)-conjugated viral ribonucleoprotein complexes (vRNPs) within infectious IAV virions and applying single-particle imaging, we tracked the uncoating process of individual IAV ...