Viral vector - Wikipedia
Viral vectors are modified viruses designed to deliver genetic material into cells. This process can be performed inside an organism or in cell culture. Viral vectors have widespread applications in basic research, agriculture, and medicine.
Viral vector platforms within the gene therapy landscape
2021年2月8日 · To review the viral vector-based gene therapy landscape, we focus on these three highly regarded vector platforms and describe mechanisms of action and their roles in treating human...
Production of adeno-associated virus vectors for - Nature
2019年9月19日 · Here, we report an improved protocol to produce serotype-independent purified AAVs economically. Using a helper-free AAV system, we purified AAVs from HEK293T cell lysates and medium by...
Viral vectored vaccines: design, development, preventive and ...
2023年4月7日 · Viral vector vaccines consist of viral particles whose genomes have been modified to contain one or more foreign genes encoding the targeted antigens. The rationale for using viruses to...
Viral vector manufacturing: Production methods & advances
2023年9月20日 · In viral vector production, the convergence of quality evidence, adherence to Good Manufacturing Practices (GMP), and the stringent regulatory demands, e.g. by the FDA, is essential for clinical trials, commercial manufacturing, and the …
Tips for viral vector production - Cytiva
2024年4月9日 · The viral vector market is highly active, and the interest in production technologies is driven by recent approvals in cell and gene therapy as well as vaccines. Here I share insights around the challenges in viral vector manufacturing.
Viral Vector Production - Beckman
“There are three basic ways to produce viral vectors: using a stable packaging cell line, using transient transfection, or using infection,” Dr. van der Loo explains. “For the latter, I’m referring to the baculovirus system developed by Robert Kotin when he was an investigator at the NIH.
Behind the Scenes: The Production of Viral Vector Vaccines
Below, we see a high-level summary of the viral vector vaccine production process: Lab — Working Cell Bank. Frozen viral master cell bank and seed stock; Upstream Process. Suspension Cell Culture in single-use bioreactors — up to 200 L; Adherent Cell Culture in multilayer flasks, packed-bed bioreactor, and single-use bioreactors; Downstream ...
Upstream Manufacturing of Gene Therapy Viral Vectors - Cell …
2018年9月26日 · Initially, the materials needed to manufacture the therapeutic viral vector must be generated. These include plasmids encoding helper-virus functions and the therapeutic gene, cell lines used to manufacture the vector, and other …
Viral Vector Upstream Processing and Clarification
2023年8月11日 · In this chapter, we review the different viral vectors used for gene therapy applications, the different cell lines and cell types used for GMP manufacturing, and the different single-use bioreactors available on the market for large-scale production.