5 天之前 · Leishmania is a vacuolar pathogen that replicates within parasitophorous vacuoles inside host phagocytes. To promote its replication, Leishmania relies on a panoply of strategies to acquire macromolecules such as lipids from host macrophages. In this study, we have evaluated the role of VAPA, an endoplasmic reticulum-resident membrane protein involved in inter-organellar lipid transport, in ...

2020年7月1日 · As recent evidence suggests that the ER vesicle-associated membrane protein–associated protein A (VAPA) recruits Kv2.1 to the clusters (17–19), we demonstrate that this peptide disrupts this interaction, without directly binding VAPA, successfully translating this molecular finding into a viable clinical strategy.

2025年1月6日 · equi ΔvapA strain, and showed that only clade-1 Vap proteins, VapA, VapK1 VapK2 and VapN, can do so. The fact that bovine and porcine Vap proteins restored intracellular replication of an equine R .

Rhodococcus equi (R. equi) is an important pulmonary pathogen in foals that often leads to the death of the horse. The bacterium harbors a virulence plasmid that encodes numerous virulence-associated proteins (Vaps) including VapA that is essential …

2016年11月9日 · 根据GenBank中杀鲑气单胞菌毒力阵列蛋白基因 (vapA)保守序列设计并合成一对特异性引物，对其特异性、灵敏度、可重复性和应用性进行评价。 结果显示，研究设计的引物具有良好的种间特异性，仅对杀鲑气单胞菌及其亚种有阳性扩增，与其他细菌不发生交叉反应。 构建的Real-time PCR标准曲线质粒拷贝数与循环阈值呈良好的线性关系，扩增所得标准曲线分别为 y =–4.8345 x +42.535，相关系数 R2 为0.998，最低检测限为34拷贝/μL，较常规PCR的灵敏度高 …

Pathogenic Rhodococcus equi release the virulence-associated protein A (VapA) within macrophage phagosomes. VapA permeabilizes phagosome and lysosome membranes and reduces acidification of both compartments. Using biophysical techniques, we found that VapA interacts with model membranes in four step …

Virulent Rhodococcus equi strains expressing virulence-associated 15-17 kDa protein (VapA) and having a large virulence plasmid (pVAPA) of 85-90 kb containing vapA gene are pathogenic for horses.

Transmembrane protein, VAMP-associated protein A (VAPA), is significantly enriched on LOs surface via direct interaction with LOs marker α V-integrin. VAPA-enriched LOs-induced pre-metastatic education transforms the bone into a fertile milieu, which supports the growth of metastatic HCC cells.

We propose that VAPA contributes to the ability of L. amazonensis to colonize macrophages by mediat-ing bi-directional transfer of lipids essential for parasite replication and virulence between the parasitophorous vacuoles and the host cell endoplasmic reticulum.

2017年7月11日 · Here we report decreased murine norovirus replication in VAPA- and VAPB-deficient cells. We characterized the role of VAPA in detail. VAPA was required for the efficiency of a step (s) in the viral replication cycle after entry of viral RNA into the cytoplasm but before the synthesis of viral minus-sense RNA.